In vivo detection of single cells by MRI
In vivo detection of single cells by MRI
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DOI：
10.1002/mrm.20718
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发表时间：
2006-02-01
影响因子：
3.3
通讯作者：
Koretsky, AP
中科院分区：
文献类型：
--
作者：
Shapiro, EM;Sharer, K;Koretsky, AP
高弛豫度细胞内造影剂的使用使MRI能够监测细胞在不同组织中的迁移和归巢，如脑、脊髓、心脏和肌肉。在细胞标记和移植之后，MRI可以检测到体内的单个细胞，并定位到组织。用绿色荧光1.63微米氧化铁颗粒和红色荧光内体标记染料双标记原代小鼠肝细胞，注射到受体小鼠的脾内。这是啮齿类动物常见的肝细胞移植范例，肝细胞作为单个细胞从脾迁移到肝脏。一个月后，动物接受了活体核磁共振检查，发现散布在肝脏各处的点状暗对比区域。灌流的固定样本和标记的肝细胞模型的MRI结合组织学评估证实存在分散的单个肝细胞移植到肝脏中。使用适当的对照来确定观察到的对比度可能是由于死亡细胞还是由于游离颗粒，结果证实对比度是由于分散的单个细胞造成的。在体内检测单个细胞打开了一系列实验的大门，例如监测罕见的细胞事件，评估干细胞归巢的动力学，以及实现转移的早期检测。
The use of high-relaxivity, intracellular contrast agents has enabled MRI monitoring of cell migration through and homing to various tissues, such as brain, spinal cord, heart, and muscle. Here it is shown that MRI can detect single cells in vivo, homing to tissue, following cell labeling and transplantation. Primary mouse hepatocytes were double-labeled with green fluorescent 1.63-mu m iron oxide particles and red fluorescent endosomal labeling dye, and injected into the spleens of recipient mice. This is a common hepatocyte transplantation paradigm in rodents whereby hepatocytes migrate from the spleen to the liver as single cells. One month later the animals underwent in vivo MRI and punctuated, dark contrast regions were detected scattered through the livers. MRI of perfused, fixed samples and labeled hepatocyte phantoms in combination with histological evaluation confirmed the presence of dispersed single hepatocytes grafted into the livers. Appropriate controls were used to determine whether the observed contrast could have been due to dead cells or free particles, and the results confirmed that the contrast was due to disperse, single cells. Detecting single cells in vivo opens the door to a number of experiments, such as monitoring rare cellular events, assessing the kinetics of stem cell homing, and achieving early detection of metastases.