Effects of increased venous pressure on albumin- and IgG-excluded volumes in skin.
Effects of increased venous pressure on albumin- and IgG-excluded volumes in skin.
复制标题
静脉压升高对皮肤中白蛋白和 IgG 排除体积的影响。
DOI：
10.1152/ajpheart.1982.242.6.h1038
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Mullins,RJ
中科院分区：
文献类型：
--
作者：
Bell,DR;Mullins,RJ
在麻醉兔身上研究了静脉压升高对皮肤间质中血浆白蛋白和 γ-免疫球蛋白 G (IgG) 排除的影响。在八只兔子中，左腿的静脉压从 7 毫米汞柱增加到 26 毫米汞柱，而右腿作为对照。采集血浆和结前腘淋巴样本 4 小时，然后采集足跟皮肤样本。分别使用 51Cr 标记的乙二胺四乙酸 (EDTA) 和 125I 标记的人血清白蛋白测量组织样本中的细胞外空间和血浆空间。蛋白质空间是通过使用火箭电免疫测定法测量内源性白蛋白和 IgG 浓度来计算的。对于对照皮肤，白蛋白和 IgG 均被排除在 50% 的间隙空间之外。静脉充血后的间隙空间增加了对照的1.4倍。间质白蛋白被洗掉，白蛋白排除体积分数没有变化。对于 IgG，血管外质量没有变化，排除体积分数有所减少。皮肤间质凝胶基质的变化与内脏器官的变化不同，与胶原蛋白含量增加一致。
The effect of increased venous pressure on the exclusion of plasma albumin and gamma-immunoglobulin G (IgG) in skin interstitium was studied in anesthetized rabbits. In eight rabbits, venous pressure in the left leg was increased from 7 to 26 mmHg, while the right leg served as a control. Samples of plasma and prenodal popliteal lymph were collected for 4 h before taking samples of heel skin. The extracellular and plasma spaces in the tissue samples were measured using 51Cr-labeled ethylenediaminetetraacetic acid (EDTA) and 125I-labeled human serum albumin, respectively. The protein spaces were calculated from measurements of endogenous albumin and IgG concentrations using rocket electroimmunoassay. For control skin, albumin and IgG were both excluded from 50% of the interstitial space. The interstitial space after venous congestion was increased 1.4 times control. There was a washout of interstitial albumin and no change in the albumin-excluded volume fraction. For IgG, there was no change in the extravascular mass and a decrease in the excluded volume fraction. Changes in the interstitial gel matrix of skin were different from those in visceral organs and consistent with a greater amount of collagen.