LEISHMANIA-MEXICANA - ENERGY-METABOLISM OF AMASTIGOTES AND PROMASTIGOTES
LEISHMANIA-MEXICANA - ENERGY-METABOLISM OF AMASTIGOTES AND PROMASTIGOTES
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DOI：
10.1016/0014-4894(82)90049-2
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发表时间：
1982-01-01
影响因子：
2.1
通讯作者：
COOMBS, GH
中科院分区：
文献类型：
--
作者：
HART, DT;COOMBS, GH
L. mexicana无鞭毛体和前鞭毛体差异显著。无鞭毛体对非酯化脂肪酸的吸收率和催化率高达10倍。在无鞭毛体转化过程和前鞭毛体生长稳定期，几乎所有可利用的外源脂肪酸都被消耗掉。脂肪酸可能是无鞭毛体重要的能量底物，前鞭毛体的利用可能反映了无鞭毛体对这些底物的需求。葡萄糖被无鞭毛体和前鞭毛体利用，但前鞭毛体的葡萄糖代谢率高达10倍。葡萄糖的摄取发生在无鞭毛体转化和前鞭毛体体外生长的整个过程中。高亚代前鞭毛体表现出显着较低的葡萄糖，但较高的氨基酸利用率比低亚代前鞭毛体。天冬酰胺，谷氨酰胺，谷氨酸，亮氨酸，赖氨酸，蛋氨酸和苏氨酸消耗大量的无鞭毛体和前鞭毛体;丙氨酸和甘氨酸排泄。脯氨酸被无鞭毛体和前鞭毛体分解代谢为CO2，但速度很低，并且在整个前鞭毛体生长过程中大量排泄。能量代谢的主要终产物是CO2和琥珀酸，这两种形式的寄生虫和有高达12%和16%的总蛋白质合成转化无鞭毛体和生长promastigotes，分别分泌。无鞭毛体和前鞭毛体中的catenol对氰化物和amytal敏感; 2-巯基乙酸酯和4-戊烯酸酯主要影响β-无鞭毛体中的氧化。
The utilization of substrates by L. mexicana amastigotes and promastigotes differed significantly. The rates of uptake and catabolism of nonesterified fatty acids were up to 10-fold higher with amastigotes. Almost all the available exogenous fatty acids were consumed during amastigote transformation and by stationary phase of promastigote growth. Fatty acids probably are important energy substrates for amastigotes; promastigote utilization may reflect the requirement for these substrates in anabolism. Glucose was utilized by amastigotes and promastigotes, but the rate of catabolism was up to 10-fold higher in promastigotes. Uptake of glucose occurred throughout amastigote transformation and growth in vitro of promastigotes. High-subpassage promastigotes exhibited markedly lower glucose, but higher amino acid utilization than low-subpassage promastigotes. Asparagine, glutamine, glutamate, leucine, lysine, methionine and threonine were consumed in large quantities by amastigotes and promastigotes; alanine and glycine were excreted. Proline was catabolized to CO2 by amastigotes and promastigotes but only at a low rate, and it was excreted in large amounts throughout promastigote growth. The major end products of energy metabolism were CO2 and succinate with both forms of the parasite and there was a secretion of up to 12 and 16% of the total protein synthesized by transforming amastigotes and growing promastigotes, respectively. Catabolism in amastigotes and promastigotes was sensitive to cyanide and amytal; 2-mercaptoacetate and 4-pentenoate primarily affected .beta.-oxidation in the amastigote.