UV-assisted treatment on hydrophobic acrylic IOLs anterior surface with methacryloyloxyethyl phosphorylcholine: Reducing inflammation and maintaining low posterior capsular opacification properties
UV-assisted treatment on hydrophobic acrylic IOLs anterior surface with methacryloyloxyethyl phosphorylcholine: Reducing inflammation and maintaining low posterior capsular opacification properties
复制标题
用甲基丙烯酰氧基乙基磷酰胆碱对疏水性丙烯酸人工晶状体前表面进行紫外线辅助治疗：减少炎症并保持低后囊膜混浊特性
DOI：
10.1016/j.msec.2017.03.029
复制
发表时间：
2017-06-01
影响因子：
7.9
通讯作者：
Xu, Zhikang
中科院分区：
文献类型：
--
作者：
Huang, Xiaodan;Luo, Chenqi;Xu, Zhikang
为了提高疏水丙烯酸人工晶状体（iol）的表面生物相容性，采用紫外辐射引发甲基丙烯酰氧乙基磷酸胆碱（MPC）接枝聚合的方法修饰其前表面。利用x射线光电子能谱（XPS）对IOL表面的化学变化进行了表征，证实了MPC的共价结合。采用场发射扫描电镜（FESEM）和原子力显微镜（AFM）分析了人工晶状体表面的形态变化。静态水接触角（WCA）测量表明，MPC接枝后，前表面亲水性增强。将未处理和前路UV-MPC iol的表面生物相容性与巨噬细胞体外粘附行为进行比较。通过观察人工晶状体光学部分和IV型胶原之间的晶状体上皮细胞（LECs）的行为来评估未经治疗、前路治疗和后路治疗的人工晶状体的后囊膜混浊程度（PCO）。结果表明，MPC修饰后的前表面可抵抗巨噬细胞的附着。然而，MPC移植到后表面增强了LECs在人工晶状体和IV型胶原之间的迁移，这可能导致严重的PCO。总之，我们的前路UV-MPC iol可以减少术后炎症，同时保持疏水性丙烯酸iol的低PCO率。(C) 2017 Elsevier B.V.版权所有
In order to enhance the surface biocompatibility of hydrophobic acrylic intraocular lenses (IOLs), the anterior surface was modified through graft polymerization with methacryloyloxyethyl phosphorylcholine (MPC) initiated by ultraviolet radiation. Chemical changes on the IOL surface were characterised by X-ray photoelectron spectroscopy (XPS) to confirm the covalent binding of MPC. Field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM) analysis were used to characterise the morphological changes to the IOL surface. Static water contact angle (WCA) measurements showed that the hydrophilicity of the anterior surface was enhanced after grafting with MPC. The surface biocompatibility of the untreated and Anterior UV-MPC IOLs was compared with the adhesion behaviour of macrophages in vitro. The degree of posterior capsule opacification (PCO) of untreated, anterior treated, and posterior treated IOLs was evaluated by observing the behaviour of lens epithelial cells (LECs) between the IOL optic portion and type IV collagen. Results suggest that the MPC modified anterior surface resisted the attachment of macrophages. However, MPC grafted onto the posterior surface enhanced the migration of LECs between the IOL optic and type IV collagen, which may result in severe PCO. In conclusion, our Anterior UV-MPC IOLs may reduce postoperative inflammation while maintaining the low PCO rates of hydrophobic acrylic IOLs. (C) 2017 Elsevier B.V. All rights reserved.