Fucoxanthin regulates adipocytokine mRNA expression in white adipose tissue of diabetic/obese KK-Ay mice
Fucoxanthin regulates adipocytokine mRNA expression in white adipose tissue of diabetic/obese KK-Ay mice
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DOI：
10.1016/j.abb.2010.05.031
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发表时间：
2010-12-01
影响因子：
3.9
通讯作者：
Miyashita, Kazuo
中科院分区：
文献类型：
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作者：
Hosokawa, Masashi;Miyashita, Tatsuya;Miyashita, Kazuo
岩藻黄素是一种在可食用的棕色海藻中发现的海洋类胡萝卜素，它可以减轻糖尿病/肥胖KK-A(y)小鼠的白色脂肪组织（WAT）体重增加和高血糖，尽管它对瘦C57BL/6J小鼠的这些参数没有影响。在喂食岩藻黄素的KK-A(y)小鼠的肛周和肠系膜WATs中，单核细胞趋化蛋白-1 （MCP-1）和肿瘤坏死因子-a （tnf - α）的mRNA表达水平与对照小鼠相比显著降低，而单核细胞趋化蛋白-1和肿瘤坏死因子-a被认为会诱导胰岛素抵抗。与KK-A(y)小鼠相比，岩藻黄素没有改变瘦C57BL/6J小鼠WAT中MCP-1和TNF-a mRNA的表达水平。岩藻黄素也降低了KK-A(y)小鼠WAT中白细胞介素-6 （IL-6）和纤溶酶原激活物抑制剂-1 mRNA的表达水平。在3T3-F442A脂肪细胞分化过程中，WAT中发现的岩藻黄嘌呤代谢物岩藻黄嘌呤可减弱tnf α诱导的MCP-1和IL-6 mRNA的过表达和蛋白质分泌。此外，岩藻黄嘌呤可降低棕榈酸刺激RAW264.7巨噬细胞样细胞中TNF-a、诱导型一氧化氮合酶（iNOS）和环氧化酶-2 (COX-2) mRNA的表达。这些研究结果表明，岩藻黄素调节WAT中参与胰岛素抵抗、iNOS和COX-2的炎性脂肪细胞因子的mRNA表达，对糖尿病/肥胖KK-A(y)小鼠有特异性作用，但对瘦C57BL/6J小鼠无特异性作用。(C) 2010爱思唯尔公司版权所有。
Fucoxanthin, a marine carotenoid found in edible brown seaweeds, attenuates white adipose tissue (WAT) weight gain and hyperglycemia in diabetic/obese KK-A(y) mice, although it does not affect these parameters in lean C57BL/6J mice. In perigonadal and mesenteric WATs of KK-A(y) mice fed fucoxanthin, mRNA expression levels of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-a (TNF-alpha), which are considered to induce insulin resistance, were markedly reduced compared to control mice. In contrast to KK-A(y) mice, fucoxanthin did not alter MCP-1 and TNF-a mRNA expression levels in the WAT of lean C57BL/6J mice. Interleukin-6 (IL-6) and plasminogen activator inhibitor-1 mRNA expression levels in WAT were also decreased by fucoxanthin in KK-A(y) mice. In differentiating 3T3-F442A adipocytes, fucoxanthinol, which is a fucoxanthin metabolite found in WAT, attenuated TNF-alpha-induced MCP-1 and IL-6 mRNA overexpression and protein secretion into the culture medium. In addition, fucoxanthinol decreased TNF-a, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) mRNA expression in RAW264.7 macrophage-like cells stimulated by palmitic acid. These findings indicate that fucoxanthin regulates mRNA expression of inflammatory adipocytokines involved in insulin resistance, iNOS, and COX-2 in WAT and has specific effects on diabetic/obese KK-A(y) mice, but not on lean C57BL/6J mice. (C) 2010 Elsevier Inc. All rights reserved.