Morphology and Phylogeny of Three Pleuronema Species (Ciliophora, Scuticociliatia) from Hangzhou Bay, China, with Description of Two New Species, P-binucleatum n. sp and P-parawiackowskii n. sp.
Morphology and Phylogeny of Three Pleuronema Species (Ciliophora, Scuticociliatia) from Hangzhou Bay, China, with Description of Two New Species, P-binucleatum n. sp and P-parawiackowskii n. sp.
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中国杭州湾三种侧膜藻（Ciliophora、Scuticociliatia）的形态和系统发育，以及两个新种 P. binucleatum n. 的描述。
DOI：
10.1111/jeu.12277
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发表时间：
2016
影响因子：
2.2
通讯作者：
Hu Xiaozhong
中科院分区：
文献类型：
--
作者：
Pan Hongbo;Hu Juxiang;Jiang Jiamei;Wang Liqing;Hu Xiaozhong
对双核侧丝藻Pleuronema binucleatum n.例如，[医]拟青霉例如，采用标准方法对采自中国杭州湾河口的P. marinum Dujardin 1841和P. marinum Dujardin 1841进行了调查。双核侧丝藻在活体条件下，其细胞大小约为90-120 × 35-50 μm，肾形体轮廓，2个大核，6 - 8个口前运动体，32-41个体运动体，膜2前段（M2 a）后端呈钩状。拟阿氏侧丝藻其特征为：体大小60-90 × 20-40 μm，椭圆形，口前动臂4 ~ 8个，体动臂20-29个，M2 a后部略弯但无钩，大核单个，香肠状。通过与其他群体的比较，认为以往的研究中存在许多错误的鉴定。并提供了海原甲藻的改进诊断：细胞长约95-180 μm，外形椭圆形;口前动肌2-4个，体动肌53-70个;第1膜和第3膜均为三排; M2 a后端直;单个收缩空泡特征性地位于体中部附近。测定了3种形态小亚基rRNA基因的序列。系统发育分析表明，该属的单系性仍然不支持。
The morphology and phylogeny of Pleuronema binucleatum n. sp., P. parawiackowskii n. sp., and P. marinum Dujardin 1841, collected from Hangzhou Bay estuary, China, were investigated using standard methods. Pleuronema binucleatum n. sp. can be identified by possessing about 90–120 × 35–50 μm cell size in vivo, reniform body outline, two macronuclei, six to eight preoral kineties, 32–41 somatic kineties, and posterior end of the anterior fragment of membranelle 2 (M2a) hook‐like. Pleuronema parawiackowskii n. sp. is characterized by the combination of the following characters: body size about 60–90 × 20–40 μm in vivo, elliptical in outline, four to eight preoral kineties, 20–29 somatic kineties, posterior portion of the M2a slightly curved but nonhooked, and single macronucleus sausage‐like. After comparison with other populations of P. marinum, it is suggested that many misidentifications exist in previous studies. And an improved diagnosis of P. marinum was supplied: cell about 95–180 μm long, elliptical in outline; 2–4 preoral kineties and 53–70 somatic kineties; both membranelle 1 and membranelle 3 three‐rowed; posterior end of the M2a straight; single contractile vacuole characteristically positioned near mid‐body. The small subunit rRNA genes of three forms were sequenced. Phylogenetic analyses indicate that the monophyly of the genus Pleuronema is still not supported.