Dynamic regulation of caveolin-1 trafficking in the germ line and embryo of Caenorhabditis elegans
Dynamic regulation of caveolin-1 trafficking in the germ line and embryo of Caenorhabditis elegans
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DOI：
10.1091/mbc.e06-03-0211
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发表时间：
2006-07-01
影响因子：
3.3
通讯作者：
Grant, Barth D.
中科院分区：
文献类型：
--
作者：
Sato, Ken;Sato, Miyuki;Grant, Barth D.
小窝蛋白是质膜上小窝形成所需的主要蛋白质组分。在这里，我们表明，贩运秀丽隐杆线虫小窝蛋白-1（CAV-1）的动态调节过程中的生殖系和胚胎的发展。在卵母细胞中，CAV-1-绿色荧光蛋白（GFP）融合蛋白存在于质膜和大囊泡（CAV-1体）中。排卵和受精后，CAV-1体与质膜融合，其方式使人想起在其他物种中描述的皮质颗粒胞吐作用。CAV-1体与质膜的融合似乎受细胞周期的调节，而不是受精本身，因为融合可以在spe-9受精突变体中进行，但被RNA干扰介导的后期促进复合物组分（EMB-27）的敲低所阻断。在胞吐作用后，大多数CAV-1-GFP在一个细胞周期内被迅速内吞和降解。卵母细胞中的CAV-1体似乎是由高尔基体以ARF-1依赖性、网格蛋白独立性机制产生的。相反，CAV-1-GFP在胚胎中的内吞和降解需要网格蛋白、发动蛋白和RAB-5。我们的研究结果表明，CAV-1的分布是高度动态的发展过程中，并提供了新的见解的排序机制，调节CAV-1本地化。
Caveolin is the major protein component required for the formation of caveolae on the plasma membrane. Here we show that trafficking of Caenorhabditis elegans caveolin-1 (CAV-1) is dynamically regulated during development of the germ line and embryo. In oocytes a CAV-1-green fluorescent protein (GFP) fusion protein is found on the plasma membrane and in large vesicles (CAV-1 bodies). After ovulation and fertilization the CAV-1 bodies fuse with the plasma membrane in a manner reminiscent of cortical granule exocytosis as described in other species. Fusion of CAV-1 bodies with the plasma membrane appears to be regulated by the advancing cell cycle, and not fertilization per se, because fusion can proceed in spe-9 fertilization mutants but is blocked by RNA interference-mediated knockdown of an anaphase-promoting complex component (EMB-27). After exocytosis, most CAV-1-GFP is rapidly endocytosed and degraded within one cell cycle. CAV-1 bodies in oocytes appear to be produced by the Golgi apparatus in an ARF-1-dependent, clathrin-independent, mechanism. Conversely endocytosis and degradation of CAV-1-GFP in embryos requires clathrin, dynamin, and RAB-5. Our results demonstrate that the distribution of CAV-1 is highly dynamic during development and provides new insights into the sorting mechanisms that regulate CAV-1 localization.