Evolution of floral meristem identity genes.: Analysis of Lolium temulentum genes related to APETALA1 and LEAFY of Arabidopsis
Evolution of floral meristem identity genes.: Analysis of Lolium temulentum genes related to APETALA1 and LEAFY of Arabidopsis
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DOI：
10.1104/pp.125.4.1788
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发表时间：
2001-04-01
期刊：
影响因子：
7.4
通讯作者：
Weigel, D
中科院分区：
文献类型：
--
作者：
Gocal, GFW;King, RW;Weigel, D
开花（花序的形成）的草毒麦是严格管制，发生迅速暴露于一个漫长的一天（LD）。在成花诱导过程中，L. temulentum与双子叶植物如拟南芥在茎尖的两个APETALA 1（AP 1）样基因LtMADS 1和LtMADS 2的表达以及L. TEMULENTUM LEAFY（LtLFY）.如原位杂交所示，LtMADS 1和LtMADS 2在营养芽顶端分生组织中表达，但在LD成花诱导的30小时内表达强烈增加。在花发育后期，LtMADS 1和LtMADS 2在小穗和小花分生组织以及颖片和外稃原基中表达。有趣的是，LtLFY检测到相当晚（LD诱导后约12天）内小穗分生组织，颖片，外稃原基。这些模式与拟南芥相反，其中LFY和AP 1在花形成早期被连续激活。LtMADS 2在转基因拟南芥中表达时，在AP 1启动子的控制下，可以部分弥补严重的ap 1 -15突变等位基因的器官数量缺陷，证实了LtMADS 2和AP 1之间的密切关系。
Flowering (inflorescence formation) of the grass Lolium temulentum is strictly regulated, occurring rapidly on exposure to a single long day (LD). During floral induction, L. temulentum differs significantly from dicot species such as Arabidopsis in the expression, at the shoot apex, of two APETALA1 (AP1)-like genes, LtMADS1 and LtMADS2, and of L. temulentum LEAFY (LtLFY). As shown by in situ hybridization, LtMADS1 and LtMADS2 are expressed in the vegetative shoot apical meristem, but expression increases strongly within 30 h of LD floral induction. Later in floral development, LtMADS1 and LtMADS2 are expressed within spikelet and floret meristems and in the glume and lemma primordia. It is interesting that LtLFY is detected quite late (about 12 d after LD induction) within the spikelet meristems, glumes, and lemma primordia. These patterns contrast with Arabidopsis, where LFY and AP1 are consecutively activated early during flower formation. LtMADS2, when expressed in transgenic Arabidopsis plants under the control of the AP1 promoter, could partially complement the organ number defect of the severe ap1-15 mutant allele, confirming a close relationship between LtMADS2 and AP1.