` Epidemiological and Molecular Studies on Lumpy Skin Disease Outbreaks in Turkey during 2014-2015
` Epidemiological and Molecular Studies on Lumpy Skin Disease Outbreaks in Turkey during 2014-2015
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DOI：
10.1111/tbed.12501
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发表时间：
2017-08-01
影响因子：
4.3
通讯作者：
Dogan, M.
中科院分区：
文献类型：
--
作者：
Sevik, M.;Dogan, M.
本研究旨在探讨土耳其爱琴海、中安纳托利亚和地中海地区块状皮肤病病毒(LSDV)的流行病学和分子特征，评估与LSDV感染相关的危险因素，并调查LSD的财务影响和库蚊的潜在作用。在LSDV的传输中。样本取自611头牛，每头牛来自不同的农场，每头牛在2014年7月和2015年6月期间被临床怀疑感染了LSDV。库蚊(Culicoidesspp.)2015年4月至6月期间被困。通过G蛋白偶联趋化因子受体基因片段的测序，对LSDV地方野毒株进行了基因特征分析。采用实时荧光定量聚合酶链式反应高分辨熔融分析技术对不同类型的羊痘病毒进行鉴定。在611只动物和库蚊中，有448只检测到病毒DNA。在448个受影响的农场中，有393个接受了调查。发病率为12.3%，病死率为6.4%。系统发育分析表明，本研究中的野外分离株与其他非洲和中东分离株聚在一起。对感染牛的分离株进行了基因分型，发现存在LSDV。广义混合线性模型显示，LSDV感染与欧洲品种、小型家庭农场和农场的湖边距离呈正相关。在接受调查的养牛场，疾病的经济成本估计为人均72.75英镑。对线粒体细胞色素氧化酶亚基I基因的序列分析表明，LSDV阳性池中的库蚊为斑点库蚊。在斑点库蚊体内检测到LSDV，提示它可能在传播LSDV中发挥了作用。此外，将受感染的动物转移到无病地区增加了LSD传播的风险。LSDV感染的控制策略应包括考虑本研究中确定的危险因素。
This study was carried out to explore epidemiological and molecular features of lumpy skin disease virus (LSDV) in the Aegean, Central Anatolian and Mediterranean regions of Turkey, to evaluate the risk factors associated with LSDV infection and to investigate the financial impact of LSD and potential role of the Culicoides spp. in the transmission of LSDV. Samples were obtained from 611 cattle, each from different farms, and each clinically suspected to be infected with LSDV during the months of July 2014 and June 2015. Culicoides spp. were trapped from April to June 2015. Genetic characterization of the local LSDV field isolates was conducted by sequencing G-protein-coupled chemokine receptor gene segment. Real-time PCR high-resolution melting analysis was used for distinguishing each type of capripoxviruses. Viral DNA was detected in 448 of the 611 animals and Culicoides midges. Three hundred and ninety-three of the 448 affected farms were surveyed. The morbidity and mortality rates detected were 12.3% and 6.4%, respectively. Phylogenetic analysis showed that the field isolates in this study were clustered together with other Africa and Middle East isolates. Genotyping of isolates from infected cattle has revealed the presence of LSDV. A generalized mixed linear model showed that there were positive associations between LSDV infection, European breeds, small-sized family-type farms and nearness of farm to a lake. The financial cost of disease presence in surveyed cattle farms was estimated to be 72.75 GBP per head. The sequence analysis of the mitochondrial cytochrome oxidase subunit I gene showed that the species of Culicoides in LSDV-positive pools was Culicoides punctatus. Detection of LSDV in Culicoides punctatus suggests that it may have played a role in transmitting LSDV. Furthermore, movement of infected animals into disease-free areas increases the risk of the transmission of LSD. Control strategies for LSDV infection should include consideration of the risk factors identified in this study.