A survey of fungal microbiota in airways of healthy volunteer subjects from Puglia (Apulia), Italy
A survey of fungal microbiota in airways of healthy volunteer subjects from Puglia (Apulia), Italy
复制标题
DOI：
10.1186/s12879-019-3718-8
复制
发表时间：
2019-01-22
影响因子：
3.7
通讯作者：
Barbaro, Maria Pia Foschino
中科院分区：
文献类型：
--
作者：
Carpagnano, Giovanna Elisiana;Susca, Antonia;Barbaro, Maria Pia Foschino
背景人类呼吸道是许多微生物的主要入口，主要是那些以空气传播颗粒如病毒和细菌实体或真菌孢子形式存在的微生物。微生物的特性与局部宿主的免疫反应相结合，将决定它们是否会被清除或粘附并定植在气道中，从而导致急性或慢性肺部疾病。与细菌一样，真菌也会导致严重的肺部疾病，但它们的感染率要低得多。肺微生物群通常使用相对侵入性的支气管镜检查程序进行采样。呼出气冷凝液（EBC）收集为肺部微生物采样提供了一种潜在的侵入性较低的替代方法。本研究试图确定一个健康的成年志愿者受试者从普利亚（普利亚），意大利.MethodsFungi多样性在27 EBC样品收集意大利成年volunteers.ResultsTen测试的主题（37，03%）原来是本真菌在EBC的队列中的真菌群落的组成。我们观察到复杂的真菌群落，其中超过10%的分离物种由Aspergillus sydowii（14.8%）和Cladosporium spp（11.11%）代表。三个科目，显示真菌存在于EBC已被诊断为呼吸道diseases.ConclusionsWe提出了一项调查的一个重要的科学领域，在其早期阶段，是真菌污染的健康受试者的气道在一个小的地理区域。此外，我们解释了我们的结果，以突出真菌在呼吸系统疾病中的潜在作用。
BackgroundThe human respiratory tract represents the major portal of entry for numerous microorganisms, primarily those occurring as airborne particles such as viral and bacterial entities, or fungal spores. Microorganism characteristics coupled with the local host immune response will determine whether they will be cleared or adhere and colonize the airways leading to acute or chronic pulmonary disease. Like bacteria, fungi can cause severe lung diseases, but their infection rates are much lower. The lung microbiota is commonly sampled using relatively invasive bronchoscopic procedures. Exhaled breath condensate (EBC) collection offers a potentially less invasive alternative for lung microbiota sampling. This study tries to determine the composition of fungal communities in a cohort of healthy adult volunteer subjects from Puglia (Apulia), Italy.MethodsFungi diversity in 27 EBC samples collected from Italian adult volunteers was investigated using conventional microbiological culturing and DNA sequencing approach.ResultsTen tested subjects (37,03%) turned out to present fungi in the EBC. We observed complex fungal communities, in which more than 10% of the isolated species are represented by Aspergillus sydowii (14,8%) and Cladosporium spp (11,11%). Three subjects that showed fungal presence in EBC have been diagnosed with a respiratory disease.ConclusionsWe present a survey of an important scientific field in its early stages that is fungal contamination of airways of healthy subjects in a small geographic area. Furthermore, we interpreted our results to highlight the potential role of fungi in the context of respiratory diseases.