Sustained release of chlorogenic acid-loaded nanomicelles alleviates bone loss in mouse periodontitis
Sustained release of chlorogenic acid-loaded nanomicelles alleviates bone loss in mouse periodontitis
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DOI：
10.1039/d2bm01099b
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发表时间：
2022-07-28
影响因子：
6.6
通讯作者：
Zhang, Lu
中科院分区：
文献类型：
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作者：
Li, Han;Xu, Jiming;Zhang, Lu
牙周炎是一种常见的慢性炎症性疾病，破坏牙周支持组织，影响口腔健康，并与系统性疾病的风险增加相关。目前，抗菌药物治疗以抗生素为主，但全身抗菌药物治疗仍存在细菌耐药性、生物利用度低和突发性释放等缺点。值得注意的是，局部使用具有缓释特性的非抗生素药物可以有效克服这些问题。研究表明，绿原酸(CGA)具有良好的抗炎和抗氧化性能。为了实现CGA的缓释，我们将CGA负载到聚(d，L-丙交酯-乙交酯)(PLGA)上，并用聚乙烯吡咯烷酮(PVP)对其进行修饰，制备了CGA-PLGA@PVP纳米胶束。结果表明，CGA-PLGA@PVP纳米胶束能延长CGA的释放时间，并能有效清除脂多糖(LPS)诱导的RAW264.7细胞中的ROS，并下调促炎细胞因子的过度表达。此外，CGA-PLGA@PVP纳米胶束局部注射后可在牙周组织中保留24小时以上，抑制牙槽骨吸收，防止牙周炎的进展，表现出良好的生物相容性。因此，CGA-PLGA@PVP纳米胶束具有良好的性能，有望成为牙周炎的一种新的治疗策略。
Periodontitis is a prevalent chronic inflammatory disease that destroys the periodontal supporting tissues, impinges on oral health, and is correlated with an increased risk of systemic disease. Currently, the main drug treatment is antibiotic therapy; however, systemic antibiotic therapy still has various drawbacks such as bacterial resistance, low bioavailability and burst release. It is noteworthy that the local use of non-antibiotic drugs with sustained release characteristics can effectively overcome these problems. It has been documented that chlorogenic acid (CGA) has good anti-inflammatory and antioxidant properties. To achieve the sustained release of CGA, we synthesized CGA-PLGA@PVP nanomicelles by loading CGA onto poly(d,l-lactide-co-glycolide) (PLGA) and modified them with polyvinylpyrrolidone (PVP) for better dispersion. The results demonstrated that CGA-PLGA@PVP nanomicelles could prolong the release time of CGA, and could not only effectively remove reactive oxygen species (ROS) but also downregulate the overexpression of proinflammatory cytokines in lipopolysaccharide (LPS)-treated RAW264.7 cells. Moreover, CGA-PLGA@PVP nanomicelles could remain in gingival tissue for more than 24 hours after local injection, inhibit alveolar bone resorption and prevent the progression of periodontitis in a mouse model, showing good biocompatibility. Therefore, CGA-PLGA@PVP nanomicelles have great properties and are expected to be a novel therapeutic strategy for periodontitis.