Morphological evidence of basal keratinocyte migration during the re-epithelialization process
Morphological evidence of basal keratinocyte migration during the re-epithelialization process
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DOI：
10.1007/s00418-008-0499-3
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发表时间：
2008-09
影响因子：
2.3
通讯作者：
A. Hosoya;Jong‐Min Lee;Sung-Won Cho;Ji-Youn Kim;N. Shinozaki;T. Shibahara;M. Shimono;Han-Sung Jung
中科院分区：
文献类型：
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作者：
A. Hosoya;Jong‐Min Lee;Sung-Won Cho;Ji-Youn Kim;N. Shinozaki;T. Shibahara;M. Shimono;Han-Sung Jung
损伤复层上皮的再生是通过角质形成细胞从伤口边缘迁移来完成的。然而，角质形成细胞在创面的迁移过程以及上皮干细胞在再上皮化过程中的作用仍有待阐明。因此，我们给胚胎小鼠注射BrdU，并在出生后两周用CO2激光照射在这些小鼠的口腔黏膜上形成上皮缺陷，用这种方法我们去除了全部上皮厚度。在未损伤的上皮中，细胞角蛋白14、p63和BrdU定位于上皮的基底层，而再生上皮内的大多数细胞呈免疫阳性。增殖细胞核抗原阴性和BrdU阳性的基底角质形成细胞，表明细胞周期缓慢，仅定位于面对真皮乳头尖端的未损伤上皮的基底层。激光照射后，除BrdU外，这些面朝乳头尖端的基底角质形成细胞呈阳性反应。这些结果表明，口腔黏膜上皮干细胞可能定位于面对真皮乳头尖端的上皮的基底层，并可能与其他基底层角质形成细胞一起在外界刺激下横向迁移。
The regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO2laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli.