Competing Endogenous RNA and Interactome Bioinformatic Analyses on Human Telomerase
Competing Endogenous RNA and Interactome Bioinformatic Analyses on Human Telomerase
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DOI：
10.1089/rej.2013.1486
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发表时间：
2014-04-01
影响因子：
2.6
通讯作者：
Giordano, Carla
中科院分区：
文献类型：
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作者：
Arancio, Walter;Pizzolanti, Giuseppe;Giordano, Carla
我们提出了一个经典的相互作用组生物信息学分析和竞争内源(CE)RNA的hTERT研究。HTERT基因编码人类端粒酶复合体的催化亚单位和限制性成分。人类端粒酶逆转录酶(HTERT)对端粒的完整性至关重要。端粒功能障碍被广泛报道与衰老、癌症和细胞衰老有关。利用BioGRID相互作用数据库(http://thebiogrid.org/))和相关分析工具如Osprey(http://biodata.mshri.on.ca/osprey/servlet/Index)和Genemania(http://genemania.org/).)对端粒酶逆转录酶基因网络进行分析使用miRWalk数据库和工具(www.ma.uni-heidelberg.de/apps/zmf/mirwalk/)，根据相互竞争的内源(Ce)RNA假说(信使[m]RNAs通过micro[mi]RNAs相互作用)，进一步分析了hTERT转录本的相互作用网络。这些分析表明Akt、核因子-kappa B(NF-kappa B)、热休克蛋白90(HSP90)、p70/p80自身抗原、14-3-3蛋白和动力蛋白在端粒功能中起作用。组蛋白乙酰化/去乙酰化和蛋白多糖代谢的作用也被提出。
We present a classic interactome bioinformatic analysis and a study on competing endogenous (ce) RNAs for hTERT. The hTERT gene codes for the catalytic subunit and limiting component of the human telomerase complex. Human telomerase reverse transcriptase (hTERT) is essential for the integrity of telomeres. Telomere dysfunctions have been widely reported to be involved in aging, cancer, and cellular senescence. The hTERT gene network has been analyzed using the BioGRID interaction database (http://thebiogrid.org/) and related analysis tools such as Osprey (http://biodata.mshri.on.ca/osprey/servlet/Index) and GeneMANIA (http://genemania.org/). The network of interaction of hTERT transcripts has been further analyzed following the competing endogenous (ce) RNA hypotheses (messenger [m] RNAs cross-talk via micro [mi] RNAs) using the miRWalk database and tools (www.ma.uni-heidelberg.de/apps/zmf/mirwalk/). These analyses suggest a role for Akt, nuclear factor-kappa B (NF-kappa B), heat shock protein 90 (HSP90), p70/p80 autoantigen, 14-3-3 proteins, and dynein in telomere functions. Roles for histone acetylation/deacetylation and proteoglycan metabolism are also proposed.