Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet
Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet
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DOI：
10.1007/7651_2018_142
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Zhao, Feng
中科院分区：
文献类型：
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作者：
Chen, Lei;Radke, Daniel;Zhao, Feng
当全厚植皮(FTG)不可行时，例如大面积创面，中厚皮片移植(STSG)是可用于全厚创面修复的标准治疗方法之一。STSG的供区可以重复获取，但STSG移植仍然受到创面愈合早期血液供应不足的限制。预血运人骨髓间充质干细胞(HMSC)片可通过提供预先形成的血管结构和血管生成因子来克服这一限制，从而加速伤口愈合和促进再生。这本书的章节提供了共培养hMSCs和内皮细胞以获得预血运的hMSC细胞片(PHCS)的方案。还提供了在大鼠自体皮肤移植模型中用于全层皮肤创面修复的预血运干细胞片的植入方案以及创面愈合效果的评估。
Split thickness skin grafts (STSGs) are one of the standard treatments available for full thickness wound repair when full thickness grafts (FTGs) are not viable, such as in the case of wounds with large surface areas. The donor sites of STSGs may be harvested repeatedly, but STSG transplants are still limited by insufficient blood supply at the early stages of wound healing. Prevascularized human mesenchymal stem cell (hMSC) sheets may accelerate wound healing and improve regeneration by providing preformed vessel structures and angiogenic factors to overcome this limitation. This book chapter provides the protocol of co-culturing hMSCs and endothelial cells to attain a prevascularized hMSC cell sheet (PHCS). The protocols for implantation of the prevascularized stem cell sheet for full thickness skin wound repair in a rat autologous skin graft model as well as the evaluation of the wound healing effects are also provided.