Quantitative microarray profiling of DNA-binding molecules
Quantitative microarray profiling of DNA-binding molecules
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DOI：
10.1021/ja0744899
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发表时间：
2007-10-10
影响因子：
15
通讯作者：
Dervan, Peter B.
中科院分区：
文献类型：
--
作者：
Puckett, James W.;Muzikar, Katy A.;Dervan, Peter B.
一个高通量的同源同源位点识别(CSI)微阵列平台查询所有524,800个10碱基对的可变点，与DNA结合的吡咯-咪唑多酰胺的DNase I足迹数据相关联。以缺氧反应元件(HRE)内的5‘-TACGT-3’为靶点的八环发夹聚酰胺产生CSI微阵列衍生的序列基序5‘-WWACGT-3’(W=A，T)。以(GAA)3重复为靶点的线性β-连接聚酰胺产生CSI微阵列衍生的序列基序5‘-AARAARWWG-3’(R=G，A)。来自每个微阵列实验的选定序列的定量DNaseI足迹使得能够对整个微阵列强度谱的K-a值进行定量预测。
A high-throughput Cognate Site Identity (CSI) microarray platform interrogating all 524 800 10-base pair variable sites is correlated to quantitative DNase I footprinting data of DNA binding pyrrole-imidazole polyamides. An eight-ring hairpin polyamide programmed to target the 5 bp sequence 5 '-TACGT-3 ' within the hypoxia response element (HRE) yielded a CSI microarray-derived sequence motif of 5 '-WWACGT-3 ' (W = A,T). A linear beta-linked polyamide programmed to target a (GAA)3 repeat yielded a CSI microarray-derived sequence motif of 5 '-AARAARWWG-3 ' (R = G,A). Quantitative DNase I footprinting of selected sequences from each microarray experiment enabled quantitative prediction of K-a values across the microarray intensity spectrum.