Glypican-3 is overexpressed in lung squamous cell carcinoma, but not in adenocarcinoma
Glypican-3 is overexpressed in lung squamous cell carcinoma, but not in adenocarcinoma
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DOI：
10.1038/modpathol.2008.37
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发表时间：
2008-07-01
期刊：
影响因子：
7.5
通讯作者：
Jothy, Serge
中科院分区：
文献类型：
--
作者：
Aviel-Ronen, Sarit;Lau, Suzanne K.;Jothy, Serge
磷脂酰肌醇蛋白聚糖-3是一种膜结合蛋白聚糖，其表达通过突变和异常蛋白表达的存在与恶性肿瘤相关。关于磷脂酰肌醇蛋白聚糖-3在肺癌中表达的报道有限，有一些证据表明其表达缺失，这表明其具有肿瘤抑制作用。我们试图在蛋白质和mRNA水平上评估磷脂酰肌醇蛋白聚糖-3在肺癌中的表达，并将其与临床、组织学和基因组特征如RAS突变状态相关联。我们使用组织芯片免疫组化研究97例患者的磷脂酰肌醇蛋白聚糖-3表达，通过定量聚合酶链反应评估143例患者的磷脂酰肌醇蛋白聚糖-3 mRNA水平，并通过等位基因特异性寡核苷酸杂交鉴定RAS突变。我们将结果与临床和组织学数据相关联。磷脂酰肌醇蛋白聚糖-3在正常肺组织中均为阴性，而在肺癌组织中有23%为阳性。蛋白和mRNA的高表达与鳞状细胞组织学有关（55%的鳞状细胞癌阳性染色，8%的腺癌阳性染色，P
Glypican-3 is a membrane-bound proteoglycan whose expression has been linked to malignancies through the existence of both mutations and aberrant protein expression. Reports on glypican-3 expression in lung cancer were limited, with some evidence for loss of expression, which suggested a tumor-suppressor role. We sought to evaluate glypican-3 expression in lung cancer at the protein and mRNA levels and correlate it with clinical, histological and genomic characteristics such as RAS mutation status. We used immunohistochemistry on tissue microarray to study glypican-3 expression in 97 patients, evaluated glypican-3 mRNA levels by quantitative polymerase chain reaction in 143 patients and identified RAS mutations by allele-specific oligonucleotide hybridization. We correlated the results with clinical and histological data. Glypican-3 immunostaining was negative in all normal lung tissues, but positive in 23% of lung carcinoma samples. High protein and mRNA expression was associated with squamous histology (positive stain in 55% of squamous cell carcinoma vs 8% of adenocarcinoma, P