Carboxyfluorescein fluorescence experiments
Carboxyfluorescein fluorescence experiments
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DOI：
10.1016/s0307-4412(00)00002-9
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发表时间：
2000-05-01
期刊：
影响因子：
--
通讯作者：
Prats, M
中科院分区：
文献类型：
--
作者：
Massou, S;Albigot, R;Prats, M
虽然荧光在生物学中是一种强大且经常使用的工具，但在生物化学课程中很少有应用于这一主题的教学。本文介绍了一些经典的羧基荧光素荧光实验，包括记录荧光光谱，氧猝灭，浓度和pH对荧光信号的影响，以及动态猝灭的Stern-Volmer表示。(C)2000年IUBMB。爱思唯尔科学有限公司出版。版权所有。
Although fluorescence is a powerful and much used tool in Biology there are very few applications for teaching this topic in Biochemistry courses. Some classical experiments in carboxyfluorescein fluorescence are presented here, including recording a fluorescence spectrum, quenching by oxygen, effect of concentration and pH on fluorescence signal, and a Stern-Volmer representation for a dynamic quenching. (C) 2000 IUBMB. Published by Elsevier Science Ltd. All rights reserved.