Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications.
Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications.
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DOI：
10.1186/1476-5926-7-7
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发表时间：
2008-10-06
期刊：
影响因子：
--
通讯作者：
Hinton DE
中科院分区：
文献类型：
--
作者：
Hardman RC;Kullman SW;Hinton DE
一种新的透明股票青（Oryzias latipes; STII），隐性的色素细胞中发现的所有色素，允许经皮成像的内部器官和组织在生活的个人。研究结果描述了在STII青鳉的非侵入性体内研究方法的发展，以及这些方法在2维和3维肝胆结构，功能和异生素反应的体内研究中的成功应用。使用亮视野、宽视野和共聚焦荧光显微镜，结合荧光探针的体内应用，在活体个体中以高分辨率（< 1 μm）在细胞水平上对肝胆系统的结构和功能特征以及外源性生物素诱导的毒性进行成像。提出的调查结果表明;（1）可以在体内以高分辨率（< 1 μm）研究/成像对外源性暴露的表型响应，（2）可以在体内定性和定量研究/成像溶质从血液到胆汁的肝胆转运，（3）可以在3维中研究这种低等脊椎动物肝脏中的肝胆结构，和（4）可以研究该模型中肝胆发育的非侵入性体内成像/描述。所描述的非侵入性体内方法是一种独特的手段，通过它来研究生物结构，功能和异生素反应与高分辨率的STII青。体内方法学也提供了未来整合分子机制的机会（例如，基因组学、蛋白质组学）与生物组织的细胞和系统水平上的表型变化。虽然我们的重点一直是肝胆系统，其他器官系统同样适合在体内研究，我们认为发现的潜力，在STII青鳉的体内研究的背景下，作为显着的。
A novel transparent stock of medaka (Oryzias latipes; STII), recessive for all pigments found in chromatophores, permits transcutaneous imaging of internal organs and tissues in living individuals. Findings presented describe the development of methodologies for non invasive in vivo investigation in STII medaka, and the successful application of these methodologies to in vivo study of hepatobiliary structure, function, and xenobiotic response, in both 2 and 3 dimensions. Using brightfield, and widefield and confocal fluorescence microscopy, coupled with the in vivo application of fluorescent probes, structural and functional features of the hepatobiliary system, and xenobiotic induced toxicity, were imaged at the cellular level, with high resolution (< 1 μm), in living individuals. The findings presented demonstrate; (1) phenotypic response to xenobiotic exposure can be investigated/imaged in vivo with high resolution (< 1 μm), (2) hepatobiliary transport of solutes from blood to bile can be qualitatively and quantitatively studied/imaged in vivo, (3) hepatobiliary architecture in this lower vertebrate liver can be studied in 3 dimensions, and (4) non invasive in vivo imaging/description of hepatobiliary development in this model can be investigated. The non-invasive in vivo methodologies described are a unique means by which to investigate biological structure, function and xenobiotic response with high resolution in STII medaka. In vivo methodologies also provide the future opportunity to integrate molecular mechanisms (e.g., genomic, proteomic) of disease and toxicity with phenotypic changes at the cellular and system levels of biological organization. While our focus has been the hepatobiliary system, other organ systems are equally amenable to in vivo study, and we consider the potential for discovery, within the context of in vivo investigation in STII medaka, as significant.