Immunopathogenesis of acute central nervous system disease produced by lymphocytic choriomeningitis virus. I. Cyclophosphamide-mediated induction by the virus-carrier state in adult mice.
Immunopathogenesis of acute central nervous system disease produced by lymphocytic choriomeningitis virus. I. Cyclophosphamide-mediated induction by the virus-carrier state in adult mice.
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DOI：
10.1084/jem.135.4.860
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发表时间：
1972-04-01
期刊：
影响因子：
--
通讯作者：
Nathanson N
中科院分区：
文献类型：
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作者：
Gilden DH;Cole GA;Monjan AA;Nathanson N
脑内注射（i.c.）后3天给予单剂量150 mg/g环磷酰胺（CY）接种淋巴细胞性脉络丛脑膜炎（LCM）病毒，对90%以上的成年BALB/c小鼠急性致死性脉络丛脑膜炎有保护作用。存活的小鼠成为持续感染的携带者，血液和大脑中的病毒滴度很高。大脑的免疫荧光检查表明，在CY诱导的载体感染最初仅限于脉络丛，室管膜，和软脑膜，但在接下来的30天逐渐蔓延到神经实质，最显着的分子层的小脑。相比之下，LCM病毒携带者小鼠产生的新生儿病毒注射，并检查成年人，显示出明显得多的脉络丛感染和更广泛的实质感染，与不同的分布在脑核，包括严重感染的小脑浦肯野细胞。
A single dose of 150 mg/g of cyclophosphamide (CY), given 3 days after intracerebral (i.c.) inoculation of lymphocytic choriomeningitis (LCM) virus, protected over 90% of adult BALB/c mice against acutely fatal choriomeningitis. Surviving mice became persistently infected carriers, with high virus titers in blood and brain. Immunofluorescent examination of the brain showed that in CY-induced carriers infection was initially confined to the choroid plexus, ependyma, and leptomeninges, but over the next 30 days gradually spread to the neural parenchyma, most notably to the molecular layer of the cerebellum. By contrast, LCM virus-carrier mice produced by neonatal virus injection and examined as adults, showed a much less marked infection of choroid plexus and much more widespread infection of parenchyma, with a different distribution among brain nuclei, including heavy infection of the Purkinje cells of the cerebellum.