Molecular Architecture of the Mouse Nervous System
Molecular Architecture of the Mouse Nervous System
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DOI：
10.1016/j.cell.2018.06.021
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发表时间：
2018-08-09
期刊：
影响因子：
64.5
通讯作者：
Linnarsson, Sten
中科院分区：
文献类型：
--
作者：
Zeisel, Amit;Hochgerner, Hannah;Linnarsson, Sten
哺乳动物的神经系统执行复杂的行为，由专门的、精确定位的和相互作用的细胞类型控制。在这里，我们使用50万个单细胞的RNA测序来创建小鼠神经系统中细胞类型的详细普查。我们在空间上映射了细胞类型，并派生了一个分层的、数据驱动的分类法。神经元是最多样化的，并按发育解剖单位和神经递质和神经肽的表达进行分组。神经元多样性由编码细胞身份、突触连通性、神经传递和膜传导的基因驱动。我们发现了七个发育边界，并与关键的谷氨酸和甘氨酸神经递质的空间分布相关联。相比之下，少突胶质细胞表现出区域特征的丧失，随后是二次多样化。这里提供的资源为理解哺乳动物神经系统的分子结构奠定了坚实的基础，并使特定细胞类型的遗传操作成为可能。
The mammalian nervous system executes complex behaviors controlled by specialized, precisely positioned, and interacting cell types. Here, we used RNA sequencing of half a million single cells to create a detailed census of cell types in the mouse nervous system. We mapped cell types spatially and derived a hierarchical, data-driven taxonomy. Neurons were the most diverse and were grouped by developmental anatomical units and by the expression of neurotransmitters and neuropeptides. Neuronal diversity was driven by genes encoding cell identity, synaptic connectivity, neurotransmission, and membrane conductance. We discovered seven developmental boundaries and correlated with the spatial distribution of key glutamate and glycine neurotransmitters. In contrast, oligodendrocytes showed a loss of regional identity followed by a secondary diversification. The resource presented here lays a solid foundation for understanding the molecular architecture of the mammalian nervous system and enables genetic manipulation of specific cell types.