Human Papillomavirus DNA in Plasma of Patients with HPV16 DNA-positive Uterine Cervical Cancer
Human Papillomavirus DNA in Plasma of Patients with HPV16 DNA-positive Uterine Cervical Cancer
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DOI：
10.1093/jjco/hyp193
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发表时间：
2010-05-01
影响因子：
2.4
通讯作者：
Masuzaki, Hideaki
中科院分区：
文献类型：
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作者：
Shimada, Takako;Yamaguchi, Naohiro;Masuzaki, Hideaki
鳞状细胞癌抗原被认为是宫颈癌最准确的血清学肿瘤标志物。然而，发现血清鳞状细胞癌抗原水平与特应性皮炎和慢性肾功能衰竭的临床严重程度显著相关。为探讨人乳头瘤病毒16（HPV 16）DNA阳性的宫颈癌患者血浆HPV 16 DNA水平及其诊断价值，对43例HPV 16阳性的宫颈上皮内瘤变和宫颈鳞癌患者进行了检测。诊断为宫颈癌20例，高度鳞状上皮内病变21例，低度鳞状上皮内病变1例，上皮内病变或恶性肿瘤阴性3例。在任何治疗之前，从所有患者收集血液样品。为了分析宫颈癌患者血浆中的人乳头瘤病毒DNA，应用人乳头瘤病毒16型（HPV 16）引物和SYBR绿色染料，采用LightCycler 480 SW 1.5型荧光定量PCR仪，对HPV 16型进行了定量PCR荧光检测。宫颈癌阳性，而正常对照组无一例。浸润性宫颈癌患者血浆人乳头瘤病毒16 DNA拷贝数高于宫颈上皮内瘤变（CIN3）、微小浸润性宫颈癌和正常人，提示治疗前抗原水平正常的患者血浆人乳头瘤病毒DNA水平可作为低浸润性宫颈癌的标志物。
The squamous cell carcinoma antigen is considered the most accurate serologic tumor marker for uterine cervical carcinoma. However, serum squamous cell carcinoma antigen levels were found to correlate significantly with clinical severity of atopic dermatitis and chronic renal failure. The present study was conducted in patients with human papillomavirus 16 DNA-positive uterine cervical cancer to determine the plasma level of human papillomavirus 16 DNA and the diagnostic values of plasma human papillomavirus DNA in these patients.Forty-three human papillomavirus 16-positive patients with cervical intraepithelial neoplasia or uterine cervical squamous cell carcinoma were recruited in this study. The diagnosis was cervical cancer in 20 patients, high-grade squamous intraepithelial lesions in 21, low-grade squamous intraepithelial lesions in 1 and negative for intraepithelial lesion or malignancy in 3 patients. Before any treatment, blood samples were collected from all patients. For analysis of human papillomavirus DNA in plasma of patients with cervical cancer, quantitative polymerase chain reaction fluorescent assay for human papillomavirus 16 was performed using human papillomavirus 16 primers and SYBR Green dye using the LightCycler 480 SW1.5 apparatus.Plasma human papillomavirus 16 DNA was detected in only 30.0% of the patients with human papillomavirus 16-positive cervical cancer and in none of normal controls. The copy number of plasma human papillomavirus 16 DNA was higher in patients with invasive cancer than in those with cervical intraepithelial neoplasia (CIN3), micro-invasive cancer and in normal individuals.These results indicated that the plasma human papillomavirus DNA level could be potentially used as a marker of low-invasive cervical cancer tumors in patients with normal squamous cell carcinoma antigen levels before treatment.