Linkage analysis of the myosin heavy chain gene in Dictyostelium discoideum using a mutation generated by homologous recombination.
Linkage analysis of the myosin heavy chain gene in Dictyostelium discoideum using a mutation generated by homologous recombination.
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使用同源重组产生的突变对盘基网柄菌中的肌球蛋白重链基因进行连锁分析。
DOI：
10.1007/bf00334396
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Spudich,JA
中科院分区：
文献类型：
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作者：
Welker,DL;DeLozanne,A;Spudich,JA
通过插入基因失活产生的突变（mhca1菌株HMM）被用于将盘状肌球蛋白重链基因（mhcA）定位到连锁组IV。与该突变相关的三个表型性状（菌落生长缓慢，突变体无法发展过去的聚集，以及存在5到10个整合载体拷贝）共分离，正如预期的那样，单个插入事件的后果。利用限制性片段长度多态性证实了这种联系。hca1突变为野生型隐性突变，为非等位基因突变，在连锁组IV上的以下位点发生突变：aggJ， aggL, couH, minA, phgBandtsgB。这项工作证明了应用标准技术的能力。对转化产生的突变体进行盘状异性遗传分析，尤其适用于分析没有经典突变或限制性片段长度多态性的基因。
A mutation (mhcA1in strain HMM) created by insertional gene inactivation was used to map theDictyostelium discoideummyosin heavy chain gene (mhcA) to linkage group IV. Three phenotypic traits associated with this mutation (slow colony growth, inability of the mutant to develop past aggregation, and the presence of five to ten integrated vector copies) cosegregated as expected for the consequences of a single insertional event. This linkage was confirmed using a restriction fragment length polymorphism. ThemhcA1mutation was recessive to wild type and was nonallelic with mutations at the following loci on linkage group IV:aggJ, aggL, couH, minA, phgBandtsgB. This work demonstrates the ability to apply standard techniques developed forD. discoideumparasexual genetic analyses to mutants generated by transformation, which is of particular relevance to analysis of genes for which no classical mutations or restriction fragment length polymorphisms are available.