Absorption and transportation of flavonoids in Herb Epimedii across Caco-2 monolayer model
Absorption and transportation of flavonoids in Herb Epimedii across Caco-2 monolayer model
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发表时间：
2009
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通讯作者：
H. Ming
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作者：
H. Ming
目的利用Caco-2细胞单层模型研究淫羊藿总黄酮的吸收和转运。方法采用Caco-2细胞单层模型研究淫羊藿苷、朝藿定A、朝藿定B、朝藿定C和宝藿苷Ⅰ在Caco-2细胞中的双向转运。采用超高效液相色谱法测定细胞培养液中5种黄酮类化合物的浓度，计算表观渗透系数（Papp）。结果淫羊藿苷、朝藿定A、朝藿定B、朝藿定C和宝藿苷Ⅰ的吸收渗透系数（PA B）分别为5.91×10-7、3.22×10-7、2.76×10-7、4.23×10-7和1.46×10-6 cm/s。除宝藿苷Ⅰ外，其余4种黄酮类化合物的渗透性均较低，且各黄酮类化合物的分泌渗透性均大于吸收渗透性。其中，宝藿苷Ⅰ的PBA是PAB的9.8倍。结论5种黄酮类化合物的肠吸收均较低，可能存在转运蛋白外排机制，单苷（如宝霍苷Ⅰ）的吸收优于双苷（如淫羊藿苷）和三苷（如朝藿定A、朝藿定B和朝藿定C）。
Objective To study the absorption and transportation of flavonoids in Herb Epimedii by using Caco-2 monolayer model. Methods Caco-2 cell monolayer model was used to study the bi-direction transport of icariin, epimedin A, epimedin B, epimedin C, and baohuoside Ⅰ. The concentration of the five flavonoids in cell culture medium was measured by UPLC and the apparent permeability coefficients (Papp) were calculated. Results The absorptive permeability coefficients (PAB) of icariin, epimedin A, epimedin B, epimedin C, and baohuoside Ⅰ were 5.91×10-7, 3.22×10-7, 2.76×10-7, 4.23×10-7, and 1.46×10-6 cm/s, respectively. Except baohuoside Ⅰ, the other four flavonodes had lower permeabilities, and the secretive permeabilities (PBA) of all the flavonoids were larger than their absorptive permeabilities. Among them, the PBA of baohuoside Ⅰ was 9.8 times as much as the PAB. Conclusion The results suggest that the intestinal absorption of the five flavonoids is lower, which might have efflux mechanism by transporters, and the absorption of monloglycoside (e.g. baohuoside Ⅰ) is better than that of diglycoside (e.g. icariin) and triglycoside (e.g. epimedin A, epimedin B, and epimedin C).