Backbone resonance assignment of the HEAT1-domain of the human eukaryotic translation initiation factor 4GI.
Backbone resonance assignment of the HEAT1-domain of the human eukaryotic translation initiation factor 4GI.
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DOI：
10.1007/s12104-013-9459-5
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发表时间：
2014-04
影响因子：
0.9
通讯作者：
Wagner G
中科院分区：
文献类型：
--
作者：
Akabayov SR;Wagner G
在蛋白质合成过程中控制翻译对于细胞增殖和分化至关重要。蛋白质翻译是由核糖体亚基上的各种蛋白质组分组装而成的。真核生物翻译起始因子4G（eIF 4G）在由eIF 4G、ATP依赖性RNA解旋酶eIF 4A和帽结合蛋白eIF 4 E组成的翻译起始复合物eIF 4F的形成中起重要作用。eIF 4G的功能之一是主要通过与eIF 4G的HEAT 1结构域结合来促进eIF 4A活性的增强。为了理解HEAT 1与eIF 4A和其他组分在翻译起始期间的相互作用，骨架分配是必不可少的。在这里，我们报告的1H，13 C和15 N骨架分配的HEAT 1结构域的人eIF 4G亚型I（eIF 4GI-HEAT 1），第一个三个HEAT结构域的eIF 4G（29 kDa）作为基础，阐明其结构和相互作用与其结合伙伴，必要的了解其生物学功能的机制。
Controlling translation during protein synthesis is crucial for cell proliferation and differentiation. Protein translation is orchestrated by an assembly of various protein components at the ribosomal subunits. The eukaryotic translation initiation factor 4G (eIF4G) plays an important role in the formation of the translation initiation complex eIF4F consisting of eIF4G, the ATP dependent RNA helicase eIF4A and the cap binding protein eIF4E. One of the functions of eIF4G is the enhancement of the activity of eIF4A facilitated mainly through binding to the HEAT1 domain of eIF4G. In order to understand the interaction of HEAT1 with eIF4A and other components during translation initiation backbone assignment is essential. Here we report the 1H, 13C and 15N backbone assignment for the HEAT1 domain of human eIF4G isoform I (eIF4GI-HEAT1), the first of three HEAT domains of eIF4G (29 kDa) as a basis for the elucidation of its structure and interactions with its binding partners, necessary for understanding the mechanism of its biological function.
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影响因子：
2.7
作者：
DELAGLIO, F;GRZESIEK, S;BAX, A
通讯作者：
BAX, A
影响因子：
2.7
作者：
JOHNSON, BA;BLEVINS, RA
通讯作者：
BLEVINS, RA
影响因子：
16
作者：
Marcotrigiano, J;Lomakin, IB;Burley, SK
通讯作者：
Burley, SK
影响因子：
64.5
作者：
Marintchev A;Edmonds KA;Marintcheva B;Hendrickson E;Oberer M;Suzuki C;Herdy B;Sonenberg N;Wagner G
通讯作者：
Wagner G
影响因子：
4.8
作者：
Korneeva, NL;First, EA;Rhoads, RE
通讯作者：
Rhoads, RE