KpnI families of long, interspersed repetitive DNAs in human and other primate genomes.
KpnI families of long, interspersed repetitive DNAs in human and other primate genomes.
复制标题
人类和其他灵长类动物基因组中长的、散布的重复 DNA 的 KpnI 家族。
DOI：
10.1093/nar/10.10.3175
复制
发表时间：
1982
影响因子：
14.9
通讯作者：
Brown,FL
中科院分区：
文献类型：
--
作者：
Shafit-Zagardo,B;Maio,JJ;Brown,FL
对所研究的所有类人灵长类动物的 DNA 进行 KpnI 限制会释放出一系列明显的片段，代表长的、散布的重复 DNA 家族，此处称为 KpnI 1.2、1.5、1.8 和 1.9 kb 家族。分离代表这些家族的人 KpnI 1.2 至 1.9 kb 片段并分别克隆到为此目的专门构建的质粒 pBK5 的 KpnI 位点。 KpnI 克隆不与克隆的灵长类 alphoid 序列交叉杂交，这表明 KpnI 家族代表与 alphoid DNA 分离且不同的序列。对克隆的 KpnI 片段的二次限制性分析证明了同一 KpnI 家族内各个成员之间的微观异质性。用 HaeIII、AluI 或 RsaI 切割卷尾猴、AGM 和人类 DNA 并与各种克隆的人类 KpnI 序列杂交的放射自显影图表明，这些差异很大的灵长类动物基因组中 KpnI 家族的组织具有显着的保守性和相对简单性。 KpnI 1.2 kb 和 1.5 kb 家族在两个重组人类基因组文库的所有噬斑中出现频率较高 (15%)。有证据表明，大部分 KpnI 家族作为高分子量片段 (>2 kb) 的簇或聚集体出现在基因组中，其中包含与低分子量片段 (1.2 至 1.9 kb) 同源的序列。
KpnI restriction of DNAs from all anthropoid primates studied releases a conspicuous series of segments representing families of long, interspersed repetitive DNAs termed here the KpnI 1.2, 1.5, 1.8 and 1.9 kb families. Human KpnI 1.2 to 1.9 kb segments representative of these families were isolated and separately cloned in the KpnI site of a plasmid pBK5, specially constructed for this purpose. The KpnI clones did not cross-hybridize with cloned, primate alphoid sequences, suggesting that the KpnI families represent sequences separate and distinct from the alphoid DNAs. Secondary restriction analyses of cloned KpnI segments demonstrated microheterogeneity among individual members within the same KpnI family. Autoradiograms of capuchin monkey, AGM and human DNA cleaved with HaeIII, AluI or RsaI and hybridized to various cloned human KpnI sequences demonstrated a remarkable conservatism and relative simplicity in the organization of the KpnI families in the genomes of these widely divergent primates. The KpnI 1.2 kb and 1.5 kb families occur in high frequency (15 %) among all plaques in two recombinant human genome libraries. Evidence is presented suggesting that the bulk of the KpnI families occur in the genome as clusters or congeries of higher molecular weight segments ( >2 kb) containing sequences homologous to the low molecular weight segments (1.2 to 1.9 kb).