Expression of a vitelline membrane protein, BmVMP23, is repressed by bmo-miR-1a-3p in silkworm, Bombyx mori
Expression of a vitelline membrane protein, BmVMP23, is repressed by bmo-miR-1a-3p in silkworm, Bombyx mori
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家蚕中卵黄膜蛋白 BmVMP23 的表达被 bmo-miR-1a-3p 抑制
DOI：
10.1016/j.febslet.2013.02.030
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发表时间：
2013-04-02
期刊：
影响因子：
3.5
通讯作者：
Zhao, Qiaoling
中科院分区：
文献类型：
--
作者：
Chen, Anli;Xia, Dingguo;Zhao, Qiaoling
先前的研究表明，BmVMP 23的3' UTR被插入片段破坏，并且BmVMP 23在“Ming”（l-e（m））致死卵中的表达下调。在本研究中，我们发现了一个与BmVMP 23的3' UTR序列完全匹配的miRNA（bmo-miR-1a-3 p）。体外共转染法检测bmo-miR-1a-3 p与BmVMP 23表达的关系。荧光素酶检测结果显示luc表达被强烈抑制。这一结果推断bmo-miR-1a-3 p可能通过与3' UTR处的靶位点互补相互作用而下调BmVMP 23的表达。(C)2013年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Previous studies have shown that the 3' UTR of BmVMP23 was destroyed by an inserted fragment, and the BmVMP23 expression was downregulated in the lethal egg of "Ming'' (l-e(m)). In this study, we found a miRNA (bmo-miR-1a-3p) that matches the 3' UTR sequence of BmVMP23 perfectly. The relationship between bmo-miR-1a-3p and the BmVMP23 expression was examined by the co-transfection in vitro. The luciferase assay result showed that luc expression was strongly repressed. This result inferred that bmo-miR-1a-3p may downregulate BmVMP23 expression via complementary interaction with the target sites at the 3' UTR. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.