Biophysical principles of choanoflagellate self-organization
Biophysical principles of choanoflagellate self-organization
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DOI：
10.1073/pnas.1909447117
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发表时间：
2020-01-21
影响因子：
11.1
通讯作者：
King, Nicole
中科院分区：
文献类型：
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作者：
Larson, Ben T.;Ruiz-Herrero, Teresa;King, Nicole
受启发的模式，多细胞的choanoflagellates，最接近的动物的亲属，我们量化的生物物理过程的形态发生的choanoflagellate Salpingoeca rosetta的玫瑰花结菌落。我们发现，尽管细胞谱系的潜在变异性，但从二维（2D）生长的早期阶段到三维生长的后期阶段，rophylaxis可再现地过渡。我们的扰动实验证明了基本分泌的细胞外基质（ECM）的玫瑰花结形态发生的根本重要性，并表明，ECM与细胞在殖民地物理约束的包装增殖细胞的相互作用，因此，控制菌落形状。一个biopepidemically启发模型，占单个细胞的大小和形状，ECM的分数，其刚度相对于细胞的模拟足以解释我们的意见，并产生一个morphospace一致的意见在一系列的多细胞后鼻孔鞭毛虫菌落。总的来说，我们的生物物理学的玫瑰花结发展的观点补充了以前的遗传观点，因此，有助于阐明细胞生物学和物理学之间的相互作用，在调节形态发生。
Inspired by the patterns of multicellularity in choanoflagellates, the closest living relatives of animals, we quantify the biophysical processes underlying the morphogenesis of rosette colonies in the choanoflagellate Salpingoeca rosetta. We find that rosettes reproducibly transition from an early stage of 2-dimensional (2D) growth to a later stage of 3D growth, despite the underlying variability of the cell lineages. Our perturbative experiments demonstrate the fundamental importance of a basally secreted extracellular matrix (ECM) for rosette morphogenesis and show that the interaction of the ECM with cells in the colony physically constrains the packing of proliferating cells and, thus, controls colony shape. Simulations of a biophysically inspired model that accounts for the size and shape of the individual cells, the fraction of ECM, and its stiffness relative to that of the cells suffices to explain our observations and yields a morphospace consistent with observations across a range of multicellular choanoflagellate colonies. Overall, our biophysical perspective on rosette development complements previous genetic perspectives and, thus, helps illuminate the interplay between cell biology and physics in regulating morphogenesis.