Phylogenetic relationships of honey bees (Hymenoptera:Apinae:Apini) inferred from nuclear and mitochondrial DNA sequence data
Phylogenetic relationships of honey bees (Hymenoptera:Apinae:Apini) inferred from nuclear and mitochondrial DNA sequence data
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DOI：
10.1016/j.ympev.2005.02.017
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发表时间：
2005-10-01
影响因子：
4.1
通讯作者：
Sheppard, WS
中科院分区：
文献类型：
--
作者：
Arias, MC;Sheppard, WS
对自然分布区内采集的10种已知蜜蜂的ND 2线粒体基因和EF 1-alpha内含子进行PCR扩增、克隆和测序。利用简约法、距离法和最大似然法对DNA序列进行分析，以探讨蜜蜂的系统发育关系。蜜蜂的遗传分析有力地支持了从形态测量分析中恢复的基本拓扑结构，将蜜蜂分为三个主要的集群：巨型蜜蜂（A。dorsata、A. binghami，和A. laboriosa）、矮蜂A. andreniformis和A. florea）和腔巢蜂（A. mellifera，意大利蜜蜂A. cerana，中华绒螯蟹A. koschevnikovi，A. nuluensis和A. nigrocincta）。然而，亚洲洞穴筑巢蜜蜂的分支包括并系类群。与同域分布的中华蜜蜂相比，从其分布区的不同部分收集的中华蜜蜂样本彼此之间的相关性较低。cerana，中华绒螯蟹A. nuluensis和A.黑星藻异域分布的西方（A。mellifera）和东部（A. cerana，中华绒螯蟹A. koschevnikovi，A. nigrocincta和A. nuluensis）腔巢物种，约18%的线粒体基因和10-15%的核内含子，表明这些群体的分化比以前估计的形态和行为研究。这后一发现necropathitates重新评估假设的起源现存的欧洲，非洲和西亚蜜蜂。A. laboriosa和A. dorsata与行为学数据一致，支持了A.劳博里奥萨。(c)2005年爱思唯尔公司All rights reserved.
Two different genomic regions (ND2 mitochondrial gene and EF1-alpha intron) were PCR amplified, cloned and sequenced for the ten known honey bee species collected within their natural range distribution. DNA sequences were analyzed using parsimony, distance and maximum likelihood methods to investigate phylogenetic relationships within Apis. The phylogenctic analyses strongly supported the basic topology recoverable from morphometric analysis, grouping the honey bees into three major clusters: giant bees (A. dorsata, A. binghami, and A. laboriosa), dwarf bees (A. andreniformis and A. florea), and cavity-nesting bees (A. mellifera, A. cerana, A. koschevnikovi, A. nuluensis, and A. nigrocincta). However, the clade of Asian cavity-nesting bees included paraphyletic taxa. Exemplars of Apis cerana collected from divergent portions of its range were less related to each other than were sympatric A. cerana, A. nuluensis, and A. nigrocincta taxa. Nucleotide sequence divergence between allopatrically distributed western (A. mellifera) and eastern (A. cerana, A. koschevnikovi, A. nigrocincta, and A. nuluensis) cavity-nesting species, around 18% for the mitochondrial gene and 10-15% for the nuclear intron, suggested an earlier divergence for these groups than previously estimated from morphometric and behavioral studies. This latter finding neccessitates reevaluation of the hypothesized origin of extant European, African, and west Asian Apis mellifera. Sequence divergence between A. laboriosa and A. dorsata was consistent with behavioral data and supports the species status of A. laboriosa. (c) 2005 Elsevier Inc. All rights reserved.