Plasma membrane H+-ATPases-mediated cytosolic proton gradient regulates pollen tube growth
Plasma membrane H+-ATPases-mediated cytosolic proton gradient regulates pollen tube growth
复制标题
质膜H-ATP酶介导的胞质质子梯度调节花粉管生长
DOI：
10.1111/jipb.12981
复制
发表时间：
2020-07-21
影响因子：
11.4
通讯作者：
Li, Hong-Ju
中科院分区：
文献类型：
--
作者：
Chen, Wei;Jia, Peng-Fei;Li, Hong-Ju
在被子植物的受精过程中，花粉管的极性生长对于精细胞的传递是必不可少的。如何调节这种两极增长一直是一个长期存在的问题。尽管缺乏遗传和细胞学支持的证据，但以前的体外药理学试验表明质子通量与花粉管生长有关。在此，我们报道了质子从花粉管尖端到柄部区域形成一个梯度，这个梯度是由三个成员的拟南芥H（+）-ATP酶（AHA）产生的。遗传分析表明，这些AHAs是必需的花粉管生长，从而提供了新的见解极性生长的调节。
The polar growth of pollen tubes is essential for the delivery of sperm cells during fertilization in angiosperms. How this polar growth is regulated has been a long-standing question. Anin vitropharmacological assay previously implicated proton flux in pollen tube growth, although genetic and cellular supporting evidence was lacking. Here, we report that protons form a gradient from the pollen tube tip to the shank region and this gradient is generated by three members ofArabidopsisH(+)-ATPases (AHAs). Genetic analysis suggested that these AHAs are essential for pollen tube growth, thus providing new insight into the regulation of polar growth.