Measurement of bronchoconstriction using whole-body plethysmograph: Comparison of freely moving versus restrained guinea pigs
Measurement of bronchoconstriction using whole-body plethysmograph: Comparison of freely moving versus restrained guinea pigs
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DOI：
10.1016/s1056-8719(98)00021-5
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发表时间：
1998-04-01
影响因子：
1.9
通讯作者：
Townley, RG
中科院分区：
文献类型：
--
作者：
Chong, BTY;Agrawal, DK;Townley, RG
我们以前用双腔体积描记器测量了豚鼠的肺功能，然而，关于双腔测量晚期哮喘反应的长期肺功能的准确性仍然存在问题。这可能受到混杂因素的影响，例如动物的压力和颈部周围项圈尺寸的差异。因此，在本研究中，我们使用单腔与双腔体盒比较了相同豚鼠中组胺诱导的支气管收缩。在双腔体箱中，比气道阻力与胸气流和鼻气流之间的时间延迟成比例，并以cmH（2）P.s.为单位进行测量。然而，在单腔体盒中，PenH单位（增强型）反映了“呼吸的努力”。“这是测量吸气和呼气之间的停顿。用DeVilbiss雾化器吸入双倍浓度的组胺（12.5 ~ 200 μ g/ml溶于生理盐水）1 min，然后吸去腔室中的残留药物1 min，再用计算机记录3 min的气道阻力。两次组胺给药之间有15 min的洗脱期。两种方法之间组胺的PC、值无统计学显著差异（F > 0.05），然而，在处理动物和消除项圈放置对支气管收缩的可能影响方面，单室体箱更容易操作。总之，数据表明组胺激发在使用sRAW单位的双腔体积描记器和使用PenH单位的单腔体积描记器中产生等效的PC，数据。(C)1998年爱思唯尔科学公司
We have previously measured pulmonary function in guinea pigs using a double-chambered plethysmograph, however, the question remains regarding the accuracy of the double-chamber to gauge the long-term pulmonary function of late asthmatic response. This may be affected by confounding factors, such as stress on the animal and differences in size of the collar around the neck. Therefore, in this study we compared histamine-induced bronchoconstriction in the same guinea pigs using a single- versus a double-chambered body box. In the double-chambered body box, the specific airway resistance is proportional to time delay between thoracic and nasal flows and measured in cmH(2)P.s. Whereas, in the single-chambered body box, PenH units (Enhanced Pause) reflect "effort of breathing." This is measured as the pause between inspiration and expiration. Doubling concentrations of histamine (12.5-200 mu g/ml dissolved in normal saline) were administered by DeVilbiss nebulizer for 1 min, followed by 1 min suction of residual drug in the chamber, and then the airway resistance was recorded by the computer for the following 3 min. There was a 15-min wash-out period between two doses of histamine. There was no statistically significant difference (F > 0.05) in the PC,,, values for histamine between the two methods, however, it was much easier to work with the single-chambered body box in terms of handling the animal and eliminating the possible influence of collar placement on the bronchoconstriction. In conclusion, the data suggests histamine challenges produce equivalent PC,,, data in both the double-chambered plethysmograph with sRAW units and single-chambered plethysmograph using the PenH units. (C) 1998 Elsevier Science Inc.