LINKED RECOGNITION OF HELPER AND CYTO-TOXIC ANTIGENIC DETERMINANTS FOR THE GENERATION OF CYTO-TOXIC LYMPHOCYTES-T
LINKED RECOGNITION OF HELPER AND CYTO-TOXIC ANTIGENIC DETERMINANTS FOR THE GENERATION OF CYTO-TOXIC LYMPHOCYTES-T
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DOI：
10.1111/j.1749-6632.1988.tb36325.x
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发表时间：
1988-08-30
影响因子：
5.2
通讯作者：
FORMAN, J
中科院分区：
文献类型：
--
作者：
CASSELL, D;FORMAN, J
这些研究提出了一个TT协作产生细胞毒性T淋巴细胞的模型。在B6 Qa-1同源小鼠中，针对Qa-1同种异体抗原的体外CTL应答需要动物首先在体内用Qa-1和第二（辅助）抗原一起致敏。CTL（Qa-1）和Th（HY）识别的抗原必须呈递在同一APC上才能成功引发。这一发现与连锁识别模型一致，其中两种分子都存在于相同的APC上，以实现两种T细胞之间的紧密接近。为了进一步测试该模型，我们证明了在辅助抗原HY不存在的情况下，如果用Th的产物IL-2共接种，小鼠可以成功地针对Qa-1引发。我们进一步表明，即使用于免疫的细胞表达HY辅助抗原，用抗L3 T4抗体处理的小鼠也不能被致敏Qa-1。两者合计，这些结果提供了进一步的支持Th和CTL之间的连接识别模型。P，其中紧密接近允许淋巴因子IL-2结合到CTL上的其受体，从而允许成功诱导CTL。P.
These studies present a model for TT collaboration for the generation of cytotoxic T lymphocytes. The in vitro CTL response against Qa-1 alloantigens in B6 Qa-1 congenic mice requires that animals are first primed in vivo with Qa-1 together with a second (helper) antigen. Both the antigen recognized by CTL (Qa-1) and Th (HY) must be presented on the same APC for successful priming. This findings is consistent with a linked recognition model whereby both molecules are presented on the same APC in order to accomplish close proximity between the two T cells. To further test this model, we demonstrated that in the absence of the helper antigen, HY, mice could be successfully primed against Qa-1 if coinoculated with a product of a Th, IL-2. We further showed that mice treated with anti-L3T4 antibodies could not be primed to Qa-1 even though the cells used for immunization expressed the HY helper antigen. Taken together, these results lend further support to a model of linked recognition between Th and CTL. P where close proximity allows for the lymphokine IL-2 to bind to its receptor on the CTL allowing for the successful induction of CTL. P.