Human corneal epithelial cells respond to ocular-pathogenic, but not to nonpathogenic-flagellin
Human corneal epithelial cells respond to ocular-pathogenic, but not to nonpathogenic-flagellin
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DOI：
10.1016/j.bbrc.2006.06.088
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发表时间：
2006-08-18
影响因子：
3.1
通讯作者：
Kinoshita, Shigeru
中科院分区：
文献类型：
--
作者：
Hozono, Yukako;Ueta, Mayumi;Kinoshita, Shigeru
在这项研究中，我们研究了TLR 5在人角膜上皮细胞（CEC）的表达，以及致病性和非致病性细菌的鞭毛蛋白触发TLR 5的功能结果。使用来自铜绿假单胞菌、鼠伤寒沙门氏菌、粘质沙雷氏菌或枯草芽孢杆菌的鞭毛蛋白。TLR 5蛋白和TLR 5特异性mRNA在人CEC上明显表达。在人角膜上皮组织中，TLR 5蛋白在组织的基底细胞和翼细胞中被检测到。眼部致病菌，即铜绿假单胞菌和S。marcescense衍生的鞭毛蛋白诱导基因激活和IL-6和IL-8产生水平显著增加。相反，眼部非致病性S.鼠伤寒沙门氏菌-和B.枯草杆菌衍生的鞭毛蛋白既不诱导基因活化，也不诱导人CEC中IL-6和IL-8的产生增加。人CEC仅对眼部病原菌的鞭毛蛋白产生应答，而对眼部非病原菌的鞭毛蛋白不产生应答，从而产生促炎细胞因子。(c)2006年爱思唯尔公司All rights reserved.
In this study, we investigated the expression of TLR5 in human corneal epithelial cells (CEC), and the functional outcome of TLR5 triggering by flagellins of pathogenic- and nonpathogenic bacteria. Flagellins derived from Pseudomonas aeruginosa, Salmonella typhimurium, Serratia marcescense or Bacillus subtilis were used. The TLR5 protein and TLR5 specific mRNA expression was evident on human CEC. In human corneal epithelium tissues, TLR5 protein was detected at the basal and wing cells of the tissues. Ocular pathogenic bacteria, namely P. aeruginosa and S. marcescense, derived flagellin induced the significantly increased level of gene activation and IL-6 and IL-8 production. In contrast, ocular nonpathogenic S. typhimurium- and B. subtilis-derived flagellin induced neither the gene activation nor the increased production of IL-6 and IL-8 in human CEC. Human CEC would respond only to flagellin derived of ocular pathogenic bacteria, but not to those derived of ocular nonpathogenic bacteria, to generate pro-inflammatory cytokines. (c) 2006 Elsevier Inc. All rights reserved.