Assessment of SARS-CoV-2 in human semen-a cohort study
Assessment of SARS-CoV-2 in human semen-a cohort study
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DOI：
10.1016/j.fertnstert.2020.05.028
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发表时间：
2020-08-01
影响因子：
6.7
通讯作者：
Bielfeld, Alexandra Petra
中科院分区：
文献类型：
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作者：
Holtmann, Nora;Edimiris, Philippos;Bielfeld, Alexandra Petra
目的：了解严重急性呼吸综合征2型冠状病毒(SARS-CoV-2)患者精液中病毒核糖核酸的存在情况，并探讨其与精液参数的相关性。设计：先导性队列研究。地点：大学医院。患者(S)：34例男性分为：1)恢复期患者(根据逆转录聚合酶链式反应或抗体证实咽拭子感染SARS-CoV-2患者)；2)阴性对照组(无抗体)；3)急性感染患者(咽拭子中检出SARS-CoV-2)。干预：采集精液和血样。主要结局指标(S)：按世界卫生组织标准进行精液质量分析。RT-PCR检测自然精液和密度梯度制备后精液中的SARS-CoV-2结果：精液标本取自无症状8~54d的康复者18例，对照组14例，新冠肺炎活动期感染者2例。RT-PCR法在精液中未检测到核糖核酸，包括两名急性新冠肺炎感染患者的精液样本。中度感染的受试者显示精子质量受损。结论：新冠肺炎轻度感染不太可能影响睾丸和附睾功能，而中度感染后精液参数似乎有损害。在康复和急性新冠肺炎阳性男子的精液中均未检测到SARS-CoV-2RNA。这表明在性接触和辅助生殖技术期间没有病毒传播，尽管需要获得进一步的数据。((C)2020年，由美国生殖医学会提供。)
Objective: To investigate the presence of viral RNA in human semen of patients with severe acute-respiratory syndrome coronavirus 2 (SARS-CoV-2) and to evaluate its presence and relevance in semen parameters.Design: Pilot cohort study.Setting: University hospital.Patient(s): Thirty-four men were distributed as: 1) patients in convalescence (patients with confirmed SARS-CoV-2 infection in pharyngeal swab according to reverse-transcription polymerase chain reaction [RT-PCR] or antibodies); 2) negative control group (no antibodies); and 3) patients with an acute infection (detection of SARS-CoV-2 in pharyngeal swab).Intervention: Semen and a blood sample were collected from each individual.Main Outcome Measure(s): Analysis of semen quality according to the World Health Organization standards. Detection of SARS-CoV-2 by RT-PCR in the native semen sample and after density gradient preparation. Confirmation of immunoglobulin (Ig) A und IgG antibodies in the blood.Result(s): Eighteen semen samples from recovered men were obtained 8-54 days after absence of symptoms, 14 from control subjects, and 2 from patients with an active COVID-19 infection. No RNA was detected by means of RT-PCR in the semen, including semen samples from two patients with an acute COVID-19 infection. Subjects with a moderate infection showed an impairment of sperm quality.Conclusion(s): A mild COVID-19 infection is not likely to affect testis and epididymis function, whereas semen parameters did seem impaired after a moderate infection. SARS-CoV-2 RNA could not be detected in semen of recovered and acute COVID-19-positive men. This suggests no viral transmission during sexual contact and assisted reproductive techniques, although further data need to be obtained. ((C) 2020 by American Society for Reproductive Medicine.)