Assessment of worldwide genetic diversity of Siberian wild rye (Elymus sibiricus L.) germplasm based on gliadin analysis.
Assessment of worldwide genetic diversity of Siberian wild rye (Elymus sibiricus L.) germplasm based on gliadin analysis.
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基于麦醇溶蛋白分析的西伯利亚野生黑麦（Elymus sibiricus L.）种质全球遗传多样性评估
DOI：
10.3390/molecules17044424
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发表时间：
2012-04-12
期刊：
影响因子：
--
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Ma X;Chen S;Zhang X;Bai S;Zhang C
E. sibiricus L.，Escherichus属的模式种是原产于北方亚洲的多年生、自花授粉和异源四倍体草，其在一些国家可以作为重要的饲料草栽培。本研究采用酸性聚丙烯酰胺凝胶电泳（Acid Polyacrylamide Gel Electrophoresis，ACGE）技术，对86份来自亚洲不同地区的西伯利亚野马种质资源进行醇溶蛋白分子标记分析，以探讨其亲缘关系。利用47条多态性带计算出的遗传相似性矩阵，其变异范围为0.108 ~ 0.952，平均值为0.373。Shannon多样性指数和Simpson多样性指数分别为0.460和0.302。聚类分析表明，青藏高原，中国和其他作为单独的组之间的加入明确划分。聚类模式可能取决于地理来源和生态适应性。基于Shannon指数的种群结构分析表明，北方半球5个地理区域内和区域间的变异比例分别为55.9%和44.1%，中国6个省区内和省区间的变异比例分别为63.4%和36.6%。这种明显的地理差异可能与气候差异和山地分布等生态地理条件有关。本研究的gladin分析结果对E.西伯利亚种质资源
E. sibiricus L., the type species of the genus Elymus, is a perennial, self-pollinating and allotetraploid grass indigenous to Northern Asia, which in some countries can be cultivated as an important forage grass. In the present study, eighty-six Elymus sibiricus accessions, mostly from different parts of Asia, were assayed by gliadin markers based on Acid Polyacrylamide Gel Electrophoresis to differentiate and explore their genetic relationships. The genetic similarity matrix was calculated by 47 polymorphic bands, which ranged from 0.108 to 0.952 with an average of 0.373. The total Shannon diversity index (Ho) and the Simpson index (He) was 0.460 and 0.302, respectively. Cluster analysis showed a clear demarcation between accessions from Qinghai-Tibetan Plateau, China and the others as separate groups. The clustering pattern was probably dependent on geographic origin and ecological adaptability of the accessions. The population structure analysis based on Shannon indices showed that the proportion of variance within and among the five geographic regions of the Northern Hemisphere was 55.9 and 44.1%, respectively, or 63.4 and 36.6% within and among six Chinese provinces. This distinct geographical divergence was perhaps depended on ecogeographical conditions such as climate difference and mountain distribution. The results of gladin analysis in this study are useful for the collection and preservation of E. sibiricus germplasm resources.
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