Fine mapping identified the gibberellin 2-oxidase gene CpDw leading to a dwarf phenotype in squash (Cucurbita pepo L.)
Fine mapping identified the gibberellin 2-oxidase gene CpDw leading to a dwarf phenotype in squash (Cucurbita pepo L.)
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精细定位确定了导致南瓜矮化表型的赤霉素 2-氧化酶基因 CpDw (Cucurbita pepo L.)
DOI：
10.1016/j.plantsci.2021.110857
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发表时间：
2021-02-27
期刊：
影响因子：
5.2
通讯作者：
Qu, Shuping
中科院分区：
文献类型：
--
作者：
Ding, Wenqi;Wang, Yunli;Qu, Shuping
矮化是南瓜的重要农艺性状，可以提高产量。在这项研究中，矮化 Cucurbita pepo L. 系 X10 的茎中细胞长度在纵向上明显短于正常藤本系 JIN234。施用外源赤霉素(GA3)后，X10的矮化状态得以恢复，表明X10可能对GA生物合成敏感。遗传分析表明，这种矮化性状是由一个完全显性基因座控制的：CpDw (Cucurbita pepo L. Dwarf)。使用来自 X10 和 JIN234 杂交的 1,300 个 F2 个体，我们将 CpDw 基因座定位到 10 号染色体上约 24.6 kb 的区域，该区域包含 5 个注释基因。 X10中Cp4.1LG10g05910.1的高表达水平和GA2ox酶的高活性表明GA 2-氧化酶基因Cp4.1LG10g05910.1是CpDw的候选基因。 Cp4.1LG10g05910.1 基因的比对揭示了两个外显子中的两个非同义单核苷酸多态性 (SNP) 突变，以及亲本系之间启动子的重要功能元件中的几个 SNP 和 InDels。葫芦属的进一步等位基因多样性分析。种质资源表明，Cp4.1LG10g05910.1可能参与C. pepo早期发育阶段的藤蔓生长，但不参与C. maxima和C. moschata。该研究为南瓜蔓长遗传调控和作物育种提供了重要的理论依据。
Dwarfism is an important agronomic trait in pumpkin that can increase yield. In this study, the dwarf Cucurbita pepo L. line X10 exhibited significantly longitudinally shorter cell length in the stem than did the normal-vine line JIN234. The dwarf stature of X10 was recovered with exogenous gibberellin (GA3) application, suggesting that X10 might be sensitive to GA biosynthesis. Genetic analysis revealed that this dwarf trait is controlled by a single completely dominant locus: CpDw (Cucurbita pepo L. Dwarf). Using 1,300 F2 individuals derived from a cross between X10 and JIN234, we mapped the CpDw locus to a region of approximately 24.6 kb on chromosome 10 that contain 5 annotated genes. The high expression level of Cp4.1LG10g05910.1 and high GA2ox enzyme activity in X10 revealed that the GA 2-oxidase gene Cp4.1LG10g05910.1 is a candidate gene for CpDw. Alignment of the Cp4.1LG10g05910.1 gene revealed two nonsynonymous single nucleotide polymorphism (SNP) mutations in the two exons, as well as several SNPs and InDels in the important functional elements of promoter between parental lines. Further allelic diversity analysis of the Cucurbita spp. germplasm resources indicated that Cp4.1LG10g05910.1 may be involved in vine growth during the early developmental stage in C. pepo but not in C. maxima or C. moschata. This study provides an important theoretical basis for the genetic regulation of vine length and crop breeding in pumpkin.