Real time in vivo imaging and measurement of serine protease activity in the mouse hippocampus using a dedicated complementary metal-oxide semiconductor imaging device
Real time in vivo imaging and measurement of serine protease activity in the mouse hippocampus using a dedicated complementary metal-oxide semiconductor imaging device
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DOI：
10.1016/j.jneumeth.2006.02.005
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发表时间：
2006-09-30
影响因子：
3
通讯作者：
Ohta, Jun
中科院分区：
文献类型：
--
作者：
Ng, David C.;Tamura, Hideki;Ohta, Jun
本研究的目的是展示互补金属氧化物半导体（CMOS）成像技术在研究小鼠大脑中的应用。通过使用专用的CMOS图像传感器，我们已经成功地在体内实时地成像和测量了脑丝氨酸蛋白酶的活性，并延长了一段时间。我们通过封装CMOS图像传感器开发了一种生物荧光成像装置，使片上成像配置成为可能。在这种配置中，不需要光学器件，因此在传感器上应用激发滤光片来取代传统荧光显微镜中发现的滤光片立方块。这种完全封装的设备厚度为350 μ m，宽2.7 mm，由176 x 144像素的阵列组成，体积足够小，可以在小鼠大脑的单个半球内进行测量，同时仍然提供足够的成像分辨率。在实验中，腹腔注射kainic酸诱导大脑丝氨酸蛋白酶活性上调。通过成像和测量检测到这种活性的荧光基质的荧光，实时捕获了这些事件。整个设备的重量不到老鼠体重的1%，有望用于研究自由移动的动物。(c) 2006 Elsevier B.V.版权所有
The aim of the present study is to demonstrate the application of complementary metal-oxide semiconductor (CMOS) imaging technology for studying the mouse brain. By using a dedicated CMOS image sensor, we have successfully imaged and measured brain serine protease activity in vivo, in real-time, and for an extended period of time. We have developed a biofluorescence imaging device by packaging the CMOS image sensor which enabled on-chip imaging configuration. In this configuration, no optics are required whereby an excitation filter is applied onto the sensor to replace the filter cube block found in conventional fluorescence microscopes. The fully packaged device measures 350 mu m thick x 2.7 mm wide, consists of an array of 176 x 144 pixels, and is small enough for measurement inside a single hemisphere of the mouse brain, while still providing sufficient imaging resolution. In the experiment, intraperitoneally injected kainic acid induced upregulation of serine protease activity in the brain. These events were captured in real time by imaging and measuring the fluorescence from a fluorogenic substrate that detected this activity. The entire device, which weighs less than 1% of the body weight of the mouse, holds promise for studying freely moving animals. (c) 2006 Elsevier B.V. All rights reserved.