The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome-derived signaling in plant development
The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome-derived signaling in plant development
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拟南芥过氧化氢酶三重突变体揭示了过氧化氢酶和过氧化物酶体衍生信号在植物发育中的重要作用
DOI：
10.1111/jipb.12649
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发表时间：
2018-07-01
影响因子：
11.4
通讯作者：
Ma, Changle
中科院分区：
文献类型：
--
作者：
Su, Tong;Wang, Pingping;Ma, Changle
过氧化氢（H2 O2）在许多代谢过程中产生。H2 O2作为一种信号分子，在植物生长发育和环境胁迫响应中起着重要作用。在拟南芥中，有三个过氧化氢酶基因，CAT 1，CAT 2和CAT 3。编码的过氧化氢酶主要是过氧化物酶体蛋白，对清除H2 O2至关重要。由于CAT 1和CAT 3在1号染色体上连锁，因此通过传统的遗传工具几乎不可能产生CAT 1/3和CAT 1/2/3突变体。在这项研究中，我们通过CRISPR/Cas9构建了cat 1/3双突变体和cat 1/2/3三突变体，以研究过氧化氢酶的作用。与野生型和cat 2/3双突变体相比，cat 1/2/3三重突变体在生理条件下表现出严重的氧化还原紊乱和生长缺陷。转录组分析显示，cat 1/2/3三重突变体的cat 2/3突变体相比，更深刻的转录反应。这些差异表达的基因参与植物的生长调节以及非生物和生物胁迫反应。此外，OXI 1（氧化信号诱导1）和几个MAPK级联基因的表达在过氧化氢酶三重突变体中发生了显着变化，表明过氧化物酶体中产生的H2 O2可以作为过氧化物酶体逆行信号。
Hydrogen peroxide (H2 O2 ) is generated in many metabolic processes. As a signaling molecule, H2 O2 plays important roles in plant growth and development, as well as environmental stress response. In Arabidopsis, there are three catalase genes, CAT1, CAT2, and CAT3. The encoded catalases are predominately peroxisomal proteins and are critical for scavenging H2 O2 . Since CAT1 and CAT3 are linked on chromosome 1, it has been almost impossible to generate cat1/3 and cat1/2/3 mutants by traditional genetic tools. In this study, we constructed cat1/3 double mutants and cat1/2/3 triple mutants by CRISPR/Cas9 to investigate the role of catalases. The cat1/2/3 triple mutants displayed severe redox disturbance and growth defects under physiological conditions compared with wild-type and the cat2/3 double mutants. Transcriptome analysis showed a more profound transcriptional response in the cat1/2/3 triple mutants compared to the cat2/3 mutants. These differentially expressed genes are involved in plant growth regulation as well as abiotic and biotic stress responses. In addition, expression of OXI1 (OXIDATIVE SIGNAL INDUCIBLE 1) and several MAPK cascade genes were changed dramatically in the catalase triple mutant, suggesting that H2 O2 produced in peroxisomes could serve as a peroxisomal retrograde signal.