Genomic and transcriptomic analyses of the medicinal fungus Antrodia cinnamomea for its metabolite biosynthesis and sexual development
Genomic and transcriptomic analyses of the medicinal fungus Antrodia cinnamomea for its metabolite biosynthesis and sexual development
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DOI：
10.1073/pnas.1417570111
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发表时间：
2014-11-04
影响因子：
11.1
通讯作者：
Li, Wen-Hsiung
中科院分区：
文献类型：
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作者：
Lu, Mei-Yeh Jade;Fan, Wen-Lang;Li, Wen-Hsiung
樟子菇是台湾的一种多孢子菇，长期以来一直被用作治疗癌症、高血压和宿醉的药物，在台湾的年市场价值超过1亿美元。我们获得了包含9254个基因的32.15 mb基因组草图。基因组本体富集和途径分析揭示了性发育和倍半萜类、三萜类、麦角甾类、对苯二酚和对苯二酚的生物合成。我们发现了在甲羟戊酸途径、萜类途径、细胞色素p450和聚酮合成酶中，菌丝体和子实体之间的基因差异表达以及242种蛋白质的差异表达，这些蛋白质可能与药用次生代谢物的产生有关。次生代谢物生物合成途径的基因表达富集，包括子实体中将羊毛甾烷转化为麦角甾烷三萜的14- α -去甲基化酶（CYP51F1）、菌丝体中合成蒽醌醇的辅酶Q （COQ）和子实体中合成抗脑啡醇的聚酮合成酶。我们的数据将有助于制定增加有用代谢物产生的策略。
Antrodia cinnamomea, a polyporus mushroom of Taiwan, has long been used as a remedy for cancer, hypertension, and hangover, with an annual market of over $100 million (US) in Taiwan. We obtained a 32.15-Mb genome draft containing 9,254 genes. Genome ontology enrichment and pathway analyses shed light on sexual development and the biosynthesis of sesquiterpenoids, triterpenoids, ergostanes, antroquinonol, and antrocamphin. We identified genes differentially expressed between mycelium and fruiting body and 242 proteins in the mevalonate pathway, terpenoid pathways, cytochrome P450s, and polyketide synthases, which may contribute to the production of medicinal secondary metabolites. Genes of secondary metabolite biosynthetic pathways showed expression enrichment for tissue-specific compounds, including 14-alpha-demethylase (CYP51F1) in fruiting body for converting lanostane to ergostane triterpenoids, coenzymes Q (COQ) for antroquinonol biosynthesis in mycelium, and polyketide synthase for antrocamphin biosynthesis in fruiting body. Our data will be useful for developing a strategy to increase the production of useful metabolites.