Metabolism and energetics in squid (Illex illecebrosus, Loligo pealei) during muscular fatigue and recovery.
Metabolism and energetics in squid (Illex illecebrosus, Loligo pealei) during muscular fatigue and recovery.
复制标题
肌肉疲劳和恢复过程中鱿鱼（Illex illecebrosus、Loligo pealei）的代谢和能量学。
DOI：
10.1152/ajpregu.1993.265.1.r157
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
R. Boutilier
中科院分区：
文献类型：
--
作者：
H. Pörtner;D. Webber;R. O'dor;R. Boutilier
在对照条件下、运动强度增加导致疲劳后以及运动后恢复期间，测定了套膜肌肉组织和从空心鱿鱼（Loligo pealei、Illex illecebrosus）中抽取的血液样本中无氧能量代谢的中间产物和终产物以及游离氨基酸的浓度。磷原消耗、章鱼碱积累（在 Illex 中比在 Loligo 中更严重）和琥珀酸积累表明无氧代谢有助于疲劳前的能量产生。脯氨酸是 Loligo 代谢的底物，正如其在地幔中的消耗所表明的那样。在这两个物种中，没有证据表明除 AMP 之外还有 ATP 分解代谢。腺苷酸和磷酸原的游离水平和总水平变化的比较表明，疲劳对 Loligo 的能量状态有较早的不利影响。 Illex 中章鱼碱形成引起的酸中毒被证明可以促进磷酸原的利用并保护 ATP 的自由能变化，从而使游泳期间的无氧代谢范围扩大，并且在 Illex 中比在 Loligo 中表达更多。在这两个物种中，磷酸-L-精氨酸、章鱼碱和L-精氨酸的总量没有减少，因此没有从地幔中释放章鱼碱，从而支持我们之前的观点，即章鱼碱和相关质子在地幔组织中循环利用。总体而言，Loligo 的代谢策略对酸碱状态的干扰要小得多。这一策略和 Illex 将酸化质子保留在组织中的替代策略可能对于保护这两个物种的血蓝蛋白功能很重要。
The concentrations of intermediate and end products of anaerobic energy metabolism and of free amino acids were determined in mantle musculature and blood sampled from cannulated, unrestrained squid (Loligo pealei, Illex illecebrosus) under control conditions, after fatigue from increasing levels of exercise, and during postexercise recovery. Phosphagen depletion, accumulation of octopine (more so in Illex than in Loligo), and accumulation of succinate indicate that anaerobic metabolism contributes to energy production before fatigue. Proline was a substrate of metabolism in Loligo, as indicated by its depletion in the mantle. In both species, there was no evidence of catabolism of ATP beyond AMP. A comparison of the changes in the free and total levels of adenylates and the phosphagen indicates an earlier detrimental effect of fatigue on the energy status in Loligo. The acidosis provoked by octopine formation in Illex was demonstrated to promote the use of the phosphagen and to protect the free energy change of ATP such that the anaerobic scope of metabolism during swimming is extended and expressed more in Illex than in Loligo. In both species, there was no decrease in the sum of phospho-L-arginine, octopine, and L-arginine, and thus no release of octopine from the mantle, thereby supporting our earlier claim that octopine and associated protons are recycled in the mantle tissue. Overall, the metabolic strategy of Loligo is much less disturbing for the acid-base status. This strategy and the alternative strategy of Illex to keep acidifying protons in the tissue may be important for the protection of hemocyanin function in the two species.