Archetypal Roles of an Abscisic Acid Receptor in Drought and Sugar Responses in Liverworts
Archetypal Roles of an Abscisic Acid Receptor in Drought and Sugar Responses in Liverworts
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DOI：
10.1104/pp.18.00761
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发表时间：
2019-01-01
期刊：
影响因子：
7.4
通讯作者：
Takezawa, Daisuke
中科院分区：
文献类型：
--
作者：
Jahan, Akida;Komatsu, Kenji;Takezawa, Daisuke
脱落酸（阿坝）通过与被子植物细胞内受体Pyrabactin resistance 1（Pyr 1）/Pyr 1-like/regulatory components of阿坝receptor（PYR/PYL/RCAR）结合来调控种子休眠和气孔关闭。编码PYR/PYL/RCAR的基因被认为出现在胚性植物的祖先中，但这些基因在非维管植物中的作用尚未确定。在地钱中，阿坝通过增加细胞内糖的积累和各种转录物如晚期胚胎发生丰富（莱亚）样基因的转录物来降低生长并增强脱水耐受性。在本研究中，我们分析了一个基因命名为MpPYL 1，这是密切相关的PYR/PYL/RCAR被子植物，在转基因苔。过表达MpPYL 1-GFP的转基因株系表现出ABA超敏生长和增强的干燥耐受性，而通过CRISPR-Cas9介导的基因组编辑产生的Mppyl 1表现出ABA不敏感生长和降低的干燥耐受性。转录组分析表明，MpPYL 1是一个主要的非生物胁迫相关基因，包括所有35 ABA诱导的LEA-like基因的调节。此外，这些转基因植物表现出对细胞外Suc的反应改变，表明阿坝和PYR/PYL/RCAR在糖反应中起作用。这些结果揭示了PYR/PYL/RCAR在阿坝反应中的重要作用，这可能是在陆地植物的共同祖先中获得的。研究结果还表明，阿坝及其受体在芽苞菊属植物营养体脱水耐性的糖响应和积累过程中具有原型作用。
Abscisic acid (ABA) controls seed dormancy and stomatal closure through binding to the intracellular receptor Pyrabactin resistance1 (Pyr1)/Pyr1-like/regulatory components of ABA receptors (PYR/PYL/RCAR) in angiosperms. Genes encoding PYR/PYL/RCAR are thought to have arisen in the ancestor of embryophytes, but the roles of the genes in nonvascular plants have not been determined. In the liverwort Marchantia polymorpha, ABA reduces growth and enhances desiccation tolerance through increasing accumulation of intracellular sugars and various transcripts such as those of Late Embryogenesis Abundant (LEA)-like genes. In this study, we analyzed a gene designated MpPYL1, which is closely related to PYR/PYL/RCAR of angiosperms, in transgenic liverworts. Transgenic lines overexpressing MpPYL1-GFP showed ABA-hypersensitive growth with enhanced desiccation tolerance, whereas Mppyl1 generated by CRISPR-Cas9-mediated genome editing showed ABA-insensitive growth with reduced desiccation tolerance. Transcriptome analysis indicated that MpPYL1 is a major regulator of abiotic stress-associated genes, including all 35 ABA-induced LEA-like genes. Furthermore, these transgenic plants showed altered responses to extracellular Suc, suggesting that ABA and PYR/PYL/RCAR function in sugar responses. The results presented here reveal an important role of PYR/PYL/RCAR in the ABA response, which was likely acquired in the common ancestor of land plants. The results also indicate the archetypal role of ABA and its receptor in sugar response and accumulation processes for vegetative desiccation tolerance in bryophytes.