Physeal bystander effects in rhabdomyosarcoma radiotherapy: experiments in a new xenograft model.
Physeal bystander effects in rhabdomyosarcoma radiotherapy: experiments in a new xenograft model.
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横纹肌肉瘤放射治疗中的骺旁观者效应：新异种移植模型中的实验。
DOI：
10.1155/2011/815190
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Damron,TimothyA
中科院分区：
文献类型：
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作者：
Horton,JasonA;Strauss,JudithA;Allen,MatthewJ;Damron,TimothyA
用于治疗儿童肌肉骨骼肉瘤的放射疗法可能会导致骨骼生长严重缺陷。几种放射防护策略已显示出保留受辐射骨骺功能的潜力，但尚未在荷瘤动物模型中进行评估。我们开发了两种生物发光的人类横纹肌肉瘤细胞系，用于在骨骼未成熟的小鼠中建立异种移植肿瘤。生物发光成像和放射线照相可以对局部放射治疗后的肿瘤生长和胫骨伸长进行连续评估。高剂量（10Gy）放射治疗显着降低了肿瘤生长速度，延长了荷瘤小鼠的中位生存期，但也导致受照射肢体显着缩短了 3.3%。暴露于较低的 2 Gy 剂量会导致肢体长度减少 4.1%，但不会延长生存期。这种新模型提供了一种临床相关的方法来测试在此背景下使用的新型放射防护剂和放射恢复策略的有效性和安全性。
Radiotherapy used in the treatment of pediatric musculoskeletal sarcomas may result in crippling defects of skeletal growth. Several radioprotective strategies have shown potential for preserving function of the irradiated epiphysis but have not been evaluated in a tumor‐bearing animal model. We developed two bioluminescent human rhabdomyosarcoma cell lines that were used to establish xenograft tumors in skeletally immature mice. Bioluminescence imaging and radiography allowed serial evaluation of tumor growth and tibial elongation following localized radiotherapy. High‐dose (10 Gy) radiotherapy significantly reduced tumor growth velocity and prolonged the median survival of tumor‐bearing mice but also resulted in a significant 3.3% shortening of the irradiated limb. Exposure to a lower, 2 Gy dose resulted in 4.1% decrease in limb length but did not extend survival. This new model provides a clinically relevant means to test the efficacy and safety of novel radioprotectant and radiorecovery strategies for use in this context.