Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc gamma RII).
Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc gamma RII).
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DOI：
10.1084/jem.166.6.1668
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发表时间：
1987-12-01
期刊：
影响因子：
--
通讯作者：
Moore KW
中科院分区：
文献类型：
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作者：
Stuart SG;Trounstine ML;Vaux DJ;Koch T;Martens CL;Mellman I;Moore KW
我们已经从单核细胞系U937中克隆并表达了编码人IgG受体（Fc γ R）的cDNA。推导的结构是一个35 kD的跨膜蛋白，与小鼠Fc[γ 2b/γ 1]受体氨基酸序列的同源性约为60%，在细胞外结构域。信号序列与小鼠Fc γ R α cDNA克隆同源，而跨膜结构域与小鼠Fc γ R β cDNA具有同源性。胞质结构域显然是独特的。胞外结构域显示与IG基因超家族的蛋白质（包括人c-fms原癌基因/CSF-1受体）具有显著的同源性。用人Fc γ R cDNA转染的小鼠Ltk-细胞表达选择性结合人IgG的细胞表面受体，并被抗Fc γ RII mAb IV.3识别。抗源自人Fc γ R序列的肽的抗体特异性染色U937细胞，但不染色携带Fc γ RII的B-淋巴母细胞样细胞系（Daudi）。这些结果将人Fc γ RII鉴定为小鼠Fc[γ 2 B/γ 1] R的同源物，并为单核细胞和B细胞上表达的Fc γ RII的异质性提供了证据。
We have cloned and expressed a cDNA encoding a human receptor for IgG (Fc gamma R) from the monocyte cell line U937. The deduced structure is a 35-kD transmembrane protein with homology to the mouse Fc[gamma 2b/gamma 1] receptor amino acid sequence of approximately 60% in the extracellular domain. The signal sequence is homologous to the mouse Fc gamma R alpha cDNA clone, while the transmembrane domain shares homology with mouse Fc gamma R beta cDNAs. The cytoplasmic domain is apparently unique. The extracellular domain shows significant homology to proteins of the Ig gene superfamily, including the human c-fms protooncogene/CSF-1 receptor. Mouse Ltk- cells transfected with the human Fc gamma R cDNA express a cell-surface receptor that selectively binds human IgG and is recognized by the anti-Fc gamma RII mAb IV.3. Antibodies against peptides derived from the human Fc gamma R sequence specifically stain U937 cells, but not an Fc gamma RII-bearing B- lymphoblastoid cell line (Daudi). These results identify the human Fc gamma RII as the homologue of mouse Fc[gamma 2b/gamma 1] R, and provide evidence for heterogeneity of Fc gamma RII expressed on monocytes and B cells.