Role of rostral medulla in serotonin-induced changes of respiratory rhythm in newborn rat brainstem-spinal cord preparations.
Role of rostral medulla in serotonin-induced changes of respiratory rhythm in newborn rat brainstem-spinal cord preparations.
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头髓质在新生大鼠脑干脊髓制剂中血清素诱导的呼吸节律变化中的作用。
DOI：
10.1016/j.neulet.2013.12.002
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Matsumoto S.
中科院分区：
文献类型：
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作者：
Makino M;Saiki C;Ide R;Matsumoto S.
血清素（5-羟色胺，5-HT）系统异常可能诱发呼吸系统疾病。我们通过横切新生大鼠脑干-脊髓制剂中面核 (nVII) 附近不同水平的制剂，检查了延髓头端的哪些区域对于 5-HT 对呼吸样神经 (fR-like) 活动频率的影响很重要。在26°C下监测2-3日龄大鼠（n=25）的脑桥-髓质-脊髓制剂的第四颈腹侧根（C4）处的fR样活性，并比较三组在横断前后应用10μM 5-HT时fR样活性的变化，其中nVII被保留（A组，n=10），部分保留（组） B，n = 7），或通过横切消除（C组，n = 8）。横断前，各组之间的静息 fR 样活性（设置为 100%）和 5-HT 的刺激作用（+101-143%）相似。横断后，所有组的静息fR样活性均增加，但5-HT对fR样活性的促进作用在A组和C组中被消除（fR样活性分别为-4%和+7%）。在 B 组中，5-HT 变得具有抑制性（fR 样活性为 -28%）。总之，在没有脑桥影响的情况下，延髓头端的一个独特部分可能介导 5-HT 对呼吸节律的抑制作用。
Abnormalities of the serotonin (5-hydroxytryptamine, 5-HT) system may induce respiratory disorders. We examined which regions in the rostral medulla are important for the effect of 5-HT on the frequency of respiratory-like nerve (fR-like) activity by transecting the preparations at different levels near the facial nucleus (nVII) in newborn rat brainstem–spinal cord preparations. The fR-like activity at the fourth cervical ventral root (C4) of the Pons–medulla–spinal cord preparations in 2–3-day-old rats (n= 25) was monitored at 26 °C, and the change in fR-like activity in response to application of 10 μM 5-HT before and after transection was compared among three groups, in which nVII was retained (group A,n= 10), partially retained (group B,n= 7), or eliminated (group C,n= 8) by the transection. Before transection, the resting fR-like activity (set to 100%) and stimulant effect of 5-HT (+101–143%) were similar among the groups. After transection, resting fR-like activity increased in all groups, but the facilitatory effects of 5-HT on the fR-like activity were abolished in groups A and C (fR-like activity of −4% and +7%, respectively). In group B, 5-HT became inhibitory (fR-like activity of −28%). In conclusion, a distinct part of the rostral medulla in the absence of pontine influences may mediate the inhibitory effects of 5-HT on the respiratory rhythm.