miR-24 Targets the Transmembrane Glycoprotein Neuropilin-1 in Human Brain Microvascular Endothelial Cells.
miR-24 Targets the Transmembrane Glycoprotein Neuropilin-1 in Human Brain Microvascular Endothelial Cells.
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DOI：
10.3390/ncrna7010009
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发表时间：
2021-02-02
期刊：
影响因子：
4.3
通讯作者：
Santulli G
中科院分区：
文献类型：
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作者：
Mone P;Gambardella J;Wang X;Jankauskas SS;Matarese A;Santulli G
神经纤毛蛋白-1是一种跨膜糖蛋白，参与了包括血管生成和免疫在内的多种过程。最近的证据还表明，它隐含在严重急性呼吸道综合征冠状病毒（SARS-CoV-2）的细胞内化中，导致2019冠状病毒病（COVID-19）。我们假设特定的microRNA可以靶向Neuropilin-1。通过结合生物信息学和功能方法，我们确定了miR-24作为神经纤毛蛋白-1转录的调节因子。由于Neuropilin-1已被证明在内皮介导的血脑屏障调节中起关键作用，我们验证了miR-24作为人脑微血管内皮细胞（hBMEC）中Neuropilin-1的功能调节剂，hBMEC是体外血脑屏障模型最合适的细胞系。
Neuropilin-1 is a transmembrane glycoprotein that has been implicated in several processes including angiogenesis and immunity. Recent evidence has also shown that it is implied in the cellular internalization of the severe acute respiratory syndrome coronavirus (SARS-CoV-2), which causes the coronavirus disease 2019 (COVID-19). We hypothesized that specific microRNAs can target Neuropilin-1. By combining bioinformatic and functional approaches, we identified miR-24 as a regulator of Neuropilin-1 transcription. Since Neuropilin-1 has been shown to play a key role in the endothelium-mediated regulation of the blood-brain barrier, we validated miR-24 as a functional modulator of Neuropilin-1 in human brain microvascular endothelial cells (hBMECs), which are the most suitable cell line for an in vitro blood–brain barrier model.