Antibacterial activity of resin composites containing surface pre-reacted glass-ionomer (S-PRG) filler
Antibacterial activity of resin composites containing surface pre-reacted glass-ionomer (S-PRG) filler
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DOI：
10.1016/j.dental.2016.06.018
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发表时间：
2016-09-01
期刊：
影响因子：
5
通讯作者：
Imazato, Satoshi
中科院分区：
文献类型：
--
作者：
Miki, Saeki;Kitagawa, Haruaki;Imazato, Satoshi
Objective.表面预反应玻璃离子（S-PRG）填料是一种为修复材料提供生物功能的技术。已报道含有S-PRG填料的树脂复合材料显示出较少的菌斑积累和减少的细菌附着。制备了不同浓度S-PRG填料的实验性树脂复合材料，并对其表面细菌生长的抑制效果以及S-PRG填料释放的离子与抗菌活性的关系进行了评价。将含有0、13.9、27.3、41.8或55.9（体积%）的S-PRG填料的五种实验树脂复合物都是捏造的将变形链球菌在固化的盘上培养18小时，以检查与实验树脂表面接触的细菌的生长。测定从各实验树脂释放到水中的Al 3+、BO 33-、F-、Na+、SiO 32-或Sr 2+的浓度。以测定的实验树脂释放浓度配制各离子的标准溶液，研究其单一离子种类对S.通过使用每种标准化溶液评价变形菌的生长。含有13.9（体积%）S-PRG填料的树脂复合材料或更大的抑制S。变异体在其表面生长。当S.在从含有S-PRG填料的树脂复合物释放的浓度为55.9（体积%）的六种离子存在下孵育变形杆菌，观察到BO 33-、F-、Al 3+和SiO 32-的细菌数量显著减少。其中BO 33-和F-对S.变形菌生长显著。我们的研究结果表明，含有S-PRG填料的树脂复合材料抑制了S。表面有变异体S-PRG填料释放出的BO 33-、F-、Al 3+、SiO 32-等离子对S.变形菌的生长，而抑制作用主要归因于BO 33-和（C）2016年牙科材料学会的释放。由爱思唯尔有限公司出版。保留所有权利。
Objective. A surface pre-reacted glass-ionomer (S-PRG) filler is a technology of interest for providing bio-functions to restorative materials. Resin composites containing S-PRG filler have been reported to show less plaque accumulation and reduced bacterial attachment. In this study, experimental resin composites containing S-PRG filler at various concentrations were fabricated, and the inhibitory effects on bacterial growth on their surface and the association of ions released from S-PRG filler with antibacterial activity were evaluated.Methods. Five kinds of experimental resin composites containing S-PRG filler at 0, 13.9, 27.3, 41.8, or 55.9 (vol.%) were fabricated. Streptococcus mutans was cultured on the cured discs for 18 h to examine the growth of bacteria in contact with the surface of the experimental resins. The concentrations of Al3+, BO33-, F-, Na+, SiO32-, or Sr2+ released from each experimental resin into water were measured. The standardized solutions of each ion were prepared at the concentrations determined to be released from the experimental resin, and their inhibitory effects of single ion species on S. mutans growth were evaluated by using each standardized solution.Results. Resin composites containing S-PRG filler at 13.9 (vol.%) or greater inhibited S. mutans growth on their surface. When S. mutans was incubated in the presence of six kinds of ions at the concentrations released from the resin composite containing S-PRG filler at 55.9 (vol.%), a significant reduction in bacterial number was observed for BO33-, F-, Al3+, and SiO32-. Among these four ions, BO33- and F- demonstrated the strongest inhibitory effect on S. mutans growth.Significance. Our findings suggest that resin composites containing S-PRG filler inhibit the growth of S. mutans on their surface. BO33-, F-, Al3+ and SiO32- released from S-PRG filler have the ability to inhibit S. mutans growth, and the inhibitory effects are mainly attributed to release of BO33- and (C) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.