Pathology of guinea pigs experimentally infected with a novel reovirus and coronavirus isolated from SARS patients
Pathology of guinea pigs experimentally infected with a novel reovirus and coronavirus isolated from SARS patients
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DOI：
10.1089/dna.2005.24.485
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发表时间：
2005-08-01
影响因子：
3.1
通讯作者：
Duan, Q
中科院分区：
文献类型：
--
作者：
Liang, LC;He, C;Duan, Q
给豚鼠接种了从 SARS 患者身上分离出来的呼肠孤病毒 (ReoV) 和冠状病毒 (SARS-CoV)，以确定它们在 SARS 病因学中的潜在作用。感染 ReoV 的动物在接种后 (PI) 第 22 天至 30 天之间死亡，而接种 ReoV 和 SARS-CoV 的动物中有 70% 在接种后第 4 天至 7 天之间死亡。当动物分别接种两种病毒时，针对 ReoV 和 SARS-CoV 的中和抗体滴度在 80 至 160 之间，而在同时感染时，抗体滴度略低于 10。接种ReoV的动物出现类似于SARS患者渗出性和渗漏性炎症的弥漫性肺泡损伤，其特点是弥漫性出血、肌瘤渗出、透明膜形成和肺泡间质II型肺细胞增生。仅在ReoV-SARS-CoV组和SARS-CoV/ReoV组中观察到肺上皮坏死、脱落和肺组织早期纤维化。其他典型的病理变化包括淋巴结和脾脏出血性坏死以及肝脏水肿变性。相反，感染SARS-CoV的豚鼠仅出现间质性肺炎。我们的实验表明，ReoV 可能是 SARS 的主要原因之一，因为同时合并感染可以复制与 SARS 患者相似的典型病理变化。该豚鼠模型可能为SARS提供有用的动物模型。
Guinea pigs were inoculated with a reovirus (ReoV) and coronavirus (SARS-CoV) isolated from SARS patients to determine their potential role in the etiology of SARS. Animals infected with ReoV died between day 22 and day 30 postinoculation ( PI) while 70% of the animals inoculated with ReoV and SARS-CoV died between day 4 to day 7 PI. The titer of neutralizing antibodies against ReoV and SARS-CoV ranged from 80 to 160 when the animals were inoculated with the two viruses, respectively, while the titer of the antibodies was just below 10 in coinfections. The animal inoculated with ReoV developed diffuse alveolar damage similar to the exudative and leakage inflammation found in SARS patients, and was characterized by diffuse hemorrhage, fibroid exudation, hyaline membrane formation, and type II pneumocytes hyperplasia in alveolar interstitia. The pulmonary epithelial necrosis, excoriation, and early fibrosis of pulmonary tissue were only observed in ReoV-SARS-CoV groups and in SARS-CoV/ReoV groups. Other typical pathological changes included hemorrhagic necrosis in lymph nodes and spleen and hydropic degeneration in the liver. On the contrary, guinea pigs infected with SARS-CoV only developed interstitial pneumonitis. Our experiment demonstrate that ReoV might be one of the primary causes of SARS, since simultaneous coinfection can duplicate the typical pathological changes similar to that of SARS patients. This guinea pig model may provide a useful animal model for SARS.