Simultaneous label-free autofluorescence-multiharmonic microscopy and beyond
Simultaneous label-free autofluorescence-multiharmonic microscopy and beyond
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DOI：
10.1063/1.5098349
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发表时间：
2019-10-01
期刊：
影响因子：
5.6
通讯作者：
Tu, Haohua
中科院分区：
文献类型：
--
作者：
Boppart, Stephen A.;You, Sixian;Tu, Haohua
在没有复杂的数据反演算法的情况下，非线性光学显微镜可以获得亚细胞分辨率和相对较大深度的图像，具有合理的内源性对比，表明真实的生物和病理状态。虽然独立的对比是通过在不同的、通常是优化的激励条件下对相同的样品平面或体积进行顺序成像得出的，但令人惊讶的是，新的激光源工程与关键生物分子的输入一起，可以实时同时获取多个内源性分子对比，以分割丰富的细胞和细胞外成分。由于这一发展允许简单的单光束单次激发和同时进行多对比外定向信号检测，由此产生的平台避免了扰动样品预处理，如荧光标记、机械切片、稀缺或相互依赖的对比度生成、样品或成像几何形状的限制以及成像内运动伪影，这些伪影限制了体内非线性光学分子成像。(C) 2019作者。
Without sophisticated data inversion algorithms, nonlinear optical microscopy can acquire images at subcellular resolution and relatively large depth, with plausible endogenous contrasts indicative of authentic biological and pathological states. Although independent contrasts have been derived by sequentially imaging the same sample plane or volume under different and often optimized excitation conditions, new laser source engineering with inputs from key biomolecules surprisingly enable real-time simultaneous acquisition of multiple endogenous molecular contrasts to segment a rich set of cellular and extracellular components. Since this development allows simple single-beam single-shot excitation and simultaneous multicontrast epidirected signal detection, the resulting platform avoids perturbative sample pretreatments such as fluorescent labeling, mechanical sectioning, scarce or interdependent contrast generation, constraints to the sample or imaging geometry, and intraimaging motion artifacts that have limited in vivo nonlinear optical molecular imaging. (C) 2019 Author(s).