Antigen presentation and T cell development in H2-M-deficient mice
Antigen presentation and T cell development in H2-M-deficient mice
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DOI：
10.1126/science.271.5253.1278
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发表时间：
1996-03-01
期刊：
影响因子：
56.9
通讯作者：
Karlsson, L
中科院分区：
文献类型：
--
作者：
FungLeung, WP;Surh, CD;Karlsson, L
HL A-DM(DM)可促进人类细胞系中主要组织相容性复合体II类分子的多肽负载。缺乏功能性H2-M的小鼠，相当于小鼠的DM，在细胞表面有正常数量的II类分子，但其中大多数与不变链衍生的片段多肽有关。这些小鼠含有大量的CD4(+)T细胞，这表明胸腺中存在阳性选择。他们的CD4(+)细胞对自身H2-M缺陷的抗原提呈细胞(APC)无反应，但对野生型APC具有高反应性。缺乏H2-M的APC不能激发野生型T细胞的增殖反应。
HLA-DM (DM) facilitates peptide loading of major histocompatibility complex class II molecules in human cell lines. Mice lacking functional H2-M, the mouse equivalent of DM, have normal amounts of class II molecules at the cell surface, but most of these are associated with invariant chain-derived CLIP peptides. These mice contain large numbers of CD4(+) T cells, which is indicative of positive selection in the thymus. Their CD4(+) cells were unresponsive to self H2-M-deficient antigen-presenting cells (APCs) but were hyperreactive to wild-type APCs. H2-M-deficient APCs failed to elicit proliferative responses from wild-type T cells.