Binding of peanut lectin to specific epithelial cell types in kidney.
Binding of peanut lectin to specific epithelial cell types in kidney.
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花生凝集素与肾脏中特定上皮细胞类型的结合。
DOI：
10.1152/ajpcell.1982.242.1.c117
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Wade,JB
中科院分区：
文献类型：
--
作者：
LeHir,M;Kaissling,B;Koeppen,BM;Wade,JB
用花生凝集素（PNA）偶联辣根过氧化物酶，在光镜和电镜下观察其与兔肾上皮膜的结合。在肾皮质和髓质的外条纹中，PNA似乎只与连接小管和集管的插层细胞的管腔膜结合。PNA也与髓质内条纹和内带的Henle环的薄降支结合。PNA的这种非常特殊的亲和力在细胞学上异质上皮的特定细胞类型的分离和表征中应该是有用的。
The binding of peanut agglutinin (PNA) to epithelial membranes of the rabbit kidney was evaluated at the light- and electron-microscope level using PNA conjugated to horseradish peroxidase. In the renal cortex and outer stripe of the medulla PNA appears to bind exclusively to the luminal membrane of intercalated cells in connecting tubules and collecting ducts. PNA also binds to the thin descending limb of the loop of Henle in the inner stripe and inner zone of the medulla. This very specific affinity of PNA should be useful in the isolation and characterization of specific cell types in cytologically heterogeneous epithelia.