Metacercariae of Heterodiplostomum lanceolatum (Trematoda: Proterodiplostomidae) found in Leptodactylus podicipinus (Anura: Leptodactylidae) from Brazil: a morphological, molecular and ecological study
Metacercariae of Heterodiplostomum lanceolatum (Trematoda: Proterodiplostomidae) found in Leptodactylus podicipinus (Anura: Leptodactylidae) from Brazil: a morphological, molecular and ecological study
复制标题
DOI：
10.1017/s0022149x19000646
复制
发表时间：
2020-01-01
影响因子：
1.6
通讯作者：
Anjos, L. A.
中科院分区：
文献类型：
--
作者：
Queiroz, M. S.;Lopez-Hernandez, D.;Anjos, L. A.
南美洲爬行动物的吸虫知之甚少，只有一个完整的生命周期。我们使用分子和形态学方法对在巴西中西部地区塞尔维里亚的沼泽池塘中收集的86只尖腹蛙（Leptodactylus podicipinus，科普，1862年）中的29只中发现的双体口样囊蚴进行了表征。后蚴被鉴定为Heterodiplostomum lanceolatum Dubois，1936（原双口虫科），这是一种罕见的在蛇中成熟的物种。在28SrDNA部分序列的系统发育分析中，H. Lanceolatum与Protrodiplostomid Crocodilicola pseudostoma（分子分歧为4.1%）和Diplostomoidea超家族的其他成员属于多分支。我们的收藏提供了深入了解这种寄生虫的生态，在感染的青蛙比未感染的青蛙，和囊蚴是更多的腹腔和后肢肌肉比在腹部肌肉，这表明未来的研究方向的传输和病理的这种protorodiplostomid。
The trematodes from South American reptiles are poorly known, with only one life cycle completely characterized. We used molecular and morphological methods to characterize diplostomoid metacercariae found in 29 of 86 pointedbelly frogs, Leptodactylus podicipinus (Cope, 1862) collected in a marsh pond in Selviria, in the central-west region of Brazil. The metacercariae were identified as Heterodiplostomum lanceolatum Dubois, 1936 (Proterodiplostomidae), a rarely reported species that matures in snakes. In phylogenetic analysis of partial sequences from 28S rDNA, H. lanceolatum fell within a polytomy with the proterodiplostomid Crocodilicola pseudostoma (molecular divergence of 4.1%) and other members of the superfamily Diplostomoidea. Our collections provide insights into the ecology of this parasite, in that infected frogs were smaller than uninfected frogs, and metacercariae were more numerous in the abdominal cavity and hindlimb muscles than in abdominal muscles, which suggests directions for future research on the transmission and pathology of this proterodiplostomid.